ESM1 Antibody | 6D4

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ESM1 Antibody | 6D4 gallery image 1

Staining of paraffin-embedded human kidney with Mouse anti Human ESM1 (MCA5191Z)

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  • ESM1 Antibody | 6D4 thumbnail image 1
  • Mouse anti Human ESM1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    6D4
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5191ZPdatasheet pdfdatasheet pdf0.1 mg
    MCA5191Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human ESM1 antibody, clone 6D4 recognizes human Endothelial cell-specific molecule 1, also known as ESM1, a 165 amino acid secreted protein involved in angiogenesis. .ESM1 is highly expressed in the lung alveolar capillary network. and is also expressed albeit at lower levels in the kidney.

      Two isoforms of ESM1 produced by alternative splicing have been described. Mouse anti Human ESM1 antibody, clone 6D4 binds to the canonical isoform 1. Binding to isoform 2 which carries significant deletions within the immunogen sequence has not been established (UniProt : Q9NQ30).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 85 - 185 of human ESM1.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      growth factor activity
      regulation of cell growth
      insulin-like growth factor binding
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional ESM1 Antibody Formats

    Formats Clone Applications Sizes available
    ESM1 Antibody : Preservative Free 6D4 P 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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