Erythropoietin Receptor Antibody

//www.bio-rad-antibodies.com/human-erythropoietin-receptor-antibody-ahp1825.html?RenderPageSpecific=1
Erythropoietin Receptor Antibody gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • Erythropoietin Receptor Antibody thumbnail image 1
  • Sheep anti Human Erythropoietin Receptor
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1825E, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1825
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Human erythropoietin receptor antibody recognizes the human Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival.

      Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function.

      Sheep anti Human erythropoietin receptor antibody (AHP1825) blocks Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies (Harris et al. 1992).

      Sheep anti Human erythropoietin receptor antibody is reported as suitable for use in immunocytochemistry and for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose. The recommended concentration for functional assays is 500ug/ml.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA50ng/ml
      Immunoprecipitation10ug/ml
      Western Blotting10ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product is suitable for use in indirect ELISA applications.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1825 detects a 49kDa band of EPOR-GST on SDS-PAGE.
    • Instructions For Use

    Additional Erythropoietin Receptor Antibody Formats

    Formats Applications Sizes available
    Erythropoietin Receptor Antibody : Purified E, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...