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Erythropoietin Alpha

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  • Recombinant Human Erythropoietin Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1538FNdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Recombinant Human erythropoietein alpha is recombinant human erythropoietein alpha, produced in CHO cells. It is a single polypeptide chain containing 166 amino acids with a predicted molecular mass of 21,000 Daltons and an apparent molecular mass of 30,400 Daltons.
    • Intended Use
    • Target Species
    • Product Form
      Recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 250ul sterile deionised water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of a carrier protein (0.1% HSA or BSA) is recommended.
    • Preparation
    • Preservative Stabilisers
      None present.
    • Activity
      The specific activity was measured by normocythaemic mice and found to be 150,000 IU/mg
    • Purity
      >98.0% by HPLC analysis and SDS-PAGE
    • Approx. Protein Concentrations
      Total protein concentration 0.2mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Citrate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of gene-specific transcription
      erythrocyte maturation
      erythropoietin receptor binding
      positive regulation of tyrosine phosphorylation of Stat5 protein
      signal transduction
      positive regulation of DNA replication
      hormone activity
      positive regulation of Ras protein signal transduction
      extracellular space
      blood circulation
      positive regulation of cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Erythropoietin Alpha Formats

    Formats Applications Sizes available
    Erythropoietin Alpha : Rec. Protein FN 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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