Erythrocyte ANKYRIN-1 Antibody

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  • Rabbit anti Human ANKYRIN-1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0580-0030E, WBdatasheet pdfdatasheet pdf1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Rabbit anti Human Erythrocyte Ankyrin antibody recognizes erythroid ankyrin (ankyrin-1), also known as ankyrin R or erythrocyte ankyrin. Ankyrin 1 is a 1881 amino acid ~220kDa cytoplasmic protein forming an attachment for a number of integral membrane proteins and cytoskeletal elements. Ankyrin-1 contains a large number of ankyrin repeats, a death domain and two ZU5 domains (UniProt: P16157).

      Multiple isoforms of ankyrin-1 are generated by alternative splicing and promptor usage, Rabbit anti Human Erythrocyte Ankyrin antibody is expected to recognize all erythrocyte isoforms of ankyrin-1 but is not expected to recognize the N-terminally truncated muscle isoforms of ankyrin-1. Additionally Rabbit anti Human Erythrocyte Ankyrin antibody is likely to cross react with ankyrin-1 from chimpanzee (Pan troglodytes) and lowland gorilla (Gorilla gorilla gorilla).

      Most ankyrins are composed of 3 structural domains; a N-terminal regulatory domain containing multiple ankyrin repeats, a central spectrin binding domain and a C-terminal regulatory domain. Ankyrin-1 is present in erythrocytes and has also been found in brain and muscle. Mutations in erythrocytic ankyrin-1 are associated with hereditary spherocytosis (Eber et al. 1996).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      In blots of human erythrocytes bands of 220 kDa were observed.
    • Instructions For Use

    Additional Erythrocyte ANKYRIN-1 Antibody Formats

    Formats Applications Sizes available
    Erythrocyte ANKYRIN-1 Antibody : Purified E, WB 1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

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