ERK5 Antibody (PrecisionAbTM Antibody) | OTI3D4

//www.bio-rad-antibodies.com/human-erk5-antibody-oti3d4-vma00383.html?RenderPageSpecific=1
ERK5 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI3D4 gallery image 1

Western blot analysis of whole cell lysates probed with ERK5 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 38 second exposure. Arrow points to ERK5 (molecular weight 115 kDa)

Enlarge
  • ERK5 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI3D4 thumbnail image 1
  • ERK5 Antibody with Control Lysate
  • Mouse anti ERK5
(Rated 0.0 out of 5 based on 0 customer reviews)
    Mouse anti human ERK5 antibody recognizes mitogen-activated protein kinase 7, also known as MAPK7, MAPK-7, BMK-1, BMK1 kinase, MAP kinase 7 or extracellular-signal-regulated kinase 5. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00383WBdatasheet pdfdatasheet pdf100 µl
      VMA00383
      VMA00383KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00383KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human ERK5 antibody recognizes extracellular signal-regulated kinase 5 (ERK5), also known as mitogen-activated protein kinase 7 (MAPK7), BMK-1, BMK1 kinase and big MAP kinase 1

        Encoded by the MAPK7 gene, ERK5 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of MAPK7 encoding two distinct isoforms have been reported (provided by RefSeq, Jul 2008).

        Mouse anti Human ERK5 antibody detects a band of 115 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti ERK5 detects a band of approximately 115 kDa in HEK293 cell lysates
        Anti ERK5 detects a band of approximately 115 kDa in HEK293 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Mouse monoclonal antibody purified by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant human ERK5 (NP_002740) produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        MyD88-dependent toll-like receptor signaling pathway
        cell differentiation
        nerve growth factor receptor signaling pathway
        cytosol
        toll-like receptor 3 signaling pathway
        protein binding
        toll-like receptor 1 signaling pathway
        MyD88-independent toll-like receptor signaling pathway
        MAP kinase activity
        Toll signaling pathway
        nucleoplasm
        cell cycle
        ATP binding
        stress-activated MAPK cascade
        toll-like receptor 4 signaling pathway
        innate immune response
        toll-like receptor 2 signaling pathway
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "ERK5" specificity.