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ERK5 antibody | OTI3D4

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Anti ERK5 Antibody, clone OTI3D4 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti ERK5 Antibody, clone OTI3D4 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti ERK5 Antibody, clone OTI3D4 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti ERK5 Antibody, clone OTI3D4 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ERK5 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 38 second exposure. Arrow points to ERK5 (molecular weight 115 kDa).
Anti ERK5 Antibody, clone OTI3D4 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of ERK5 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti ERK5 antibody (VMA00383) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 163 second and (B) 30 second exposure.
Anti ERK5 Antibody, clone OTI3D4 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of ERK5 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti ERK5

Product Type
PrecisionAb Monoclonal
Clone
OTI3D4
Isotype
IgG2a
Format
Purified
Specificity
ERK5

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Product Code Applications Pack Size List Price Your Price Qty
VMA00383
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human ERK5 antibody, clone OTI3D4 recognizes extracellular signal-regulated kinase 5 (ERK5), also known as mitogen-activated protein kinase 7 (MAPK7), BMK-1, BMK1 kinase and big MAP kinase 1

Encoded by the MAPK7 gene, ERK5 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of MAPK7 encoding two distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human ERK5 antibody detects a band of 115 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ERK5 detects a band of approximately 115 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ERK5 (NP_002740) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.35 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q13164
Entrez Gene
MAPK7
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004707 MAP kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0007049 cell cycle
GO:0008063 Toll signaling pathway
GO:0030154 cell differentiation
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade

VMA00383

160322

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