ERK1 (pTyr204) Antibody

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ERK1 (pTyr204) Antibody gallery image 1

Western blot analysis of extracts from SK-BR-3 cells untreated or treated with EGF and Insulin using Rabbit anti ERK1 (pTyr204) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ERK1 (pTyr204) antibody

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  • Rabbit anti ERK1 (pTyr204)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2607P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2607
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti ERK1 (pTyr204) antibody recognizes ERK1, also known as MAPK3, when phosphorylated at tyrosine 204. ERK1 is a member of the Mitogen Activated Protein Kinase (MAPK) family. MAPKs constitute a diverse set of signaling pathways. In the case of ERK, the cascade is initiated by binding activation of a membrane bound receptor which causes activation of a small GTPase, RAS, which in turn activates the protein kinase activity of the RAF protein. RAF in turn phosphorylates and activates MEK. MEK induces phosphorylation of ERK1 at residues threonine 202 and tyrosine 204. Phosphorylation of the residues corresponds to activation of ERK1 and mediation of its diverse biological functions including cell growth, differentiation, motility, and adhesion via its effects on transcription, translation, cytoskeletal rearrangement and cross talk with other signaling pathways.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/100
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional ERK1 (pTyr204) Antibody Formats

    Formats Applications Sizes available
    ERK1 (pTyr204) Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

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