Goat anti Human ERK1 antibody detects an epitope within the N-terminal (NT) of extracellular signal-regulated kinase 1 (ERK1). ERK1 is a ~45 kDa protein involved in the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. ERK1 is a member of the MAP kinase (MAPK) family, and is activated by MAP/ERK kinase 1 (MEK1) and MEK2. ERK1 and ERK2, are emerging as important regulators of neuronal responses to both functional and pathologic stimuli.
Purified IgG - liquid
Antisera to ERK 1 were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Sodium Azide (NaN3)
Bovine Serum Albumin
Peptide with sequence C-GGEPRRTEGVGP from the N-terminal of ERK 1 .
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1062 detects a band of approximately 45kDa in human brain, HEK293 and A431lysates.