ERK1 Antibody | 3C9

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ERK1 Antibody | 3C9 gallery image 1

Western blot analysis of ERK1 expression in A-431 using Mouse anti Human ERK1:Azide Free (MCA3361Z)

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ERK1 Antibody | 3C9 gallery image 2

Immunofluorescence showing ERK1 in HeLa cells using Mouse anti Human ERK1:Azide Free (MCA3361Z)

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ERK1 Antibody | 3C9 gallery image 3

Staining of paraffin embedded human placenta with Mouse anti Human ERK1:Azide Free (MCA3361Z)

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ERK1 Antibody | 3C9 gallery image 4

Sandwich ELISA analysis of ERK1 binding using Mouse anti Human ERK1 antibody, clone 3C9 (MCA3361Z) as a capture reagent and biotinylated Mouse anti Human ERK1 anribody, clone 3D6 (MCA4808Z) as a detection reagent with purified human ERK1 as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A neat serum sample (green) is indicated

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  • Mouse anti Human ERK1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3C9
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3361ZIF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3361Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human ERK1 antibody, clone 3C9 human Mitogen-activated protein kinase 3, also known as ERK1, ERT2, MAP kinase isoform p44 or Microtubule-associated protein 2 kinase. ERK1 is a 335 amino acid ~44kDa serine treonine kinase and essential component of the MAP kinase signal transduction pathway (UniProt: P27361).

      Mouse anti Human ERK1 antibody, clone 3C9 has been sucessfully used as a capture reagent in the development of a sensitive Sandwich ELISA with Mouse anti Human ERK1 antibody, clone 3D6 as a detection reagent with purified human ERK1 as antigen for the generation of the standard curve, for the detection of ERK1 in human samples.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional ERK1 Antibody Formats

    Formats Clone Applications Sizes available
    ERK1 Antibody : Preservative Free 3C9 IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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          Recommended Positive Controls

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