ErbB3 Antibody (PrecisionAbTM Antibody)

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ErbB3 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with ErbB3 antibody followed by detection with HRP conjugated Rabbit anti Goat IgG (H/L) (1/10,000, 5160-2504) and visualized on the ChemiDoc MP with 35 second exposure. Arrow points to ErbB3 (molecular weight 185 kDa)

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  • ErbB3 Antibody with Control Lysate
  • Goat anti ErbB3
  • ErbB3 Antibody with Control Lysate
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    ErbB3 antibody recognizes receptor tyrosine-protein kinase ErbB-3, also known as proto-oncogene-like protein c-ErbB-3 tyrosine kinase-type cell surface receptor HER3. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00003WBdatasheet pdfdatasheet pdf100 µl
      VPA00003
      VPA00003KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00003K
      VPA00003KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00003KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human ErbB3 antibody recognizes the human receptor tyrosine-protein kinase ErbB-3, also known as proto-oncogene-like protein c-ErbB-3 or tyrosine kinase-type cell surface receptor HER3. ErbB3 is a 1342 amino acid single pass type 1 membrane glycoprotein, a tyrosine protein kinase containing a single protein kinase domain, originally considered inactive (Citri et al. 2003). However, subsequent studies have demonstrated that the kinase domain is competent to bind ATP and retains sufficient activity for the catalysis of autophosphorylation (Shi et al. 2010). Multiple isoforms of ErbB3, generated by alternative splicing have been described (Katoh et al. 1993).

        Goat anti Human Erb3 antibody is generated against a peptide located within the C-terminal region and is expected to recognize membrane bound isoforms 1,4 and 5 but not the short isoforms 2 and 3. ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and has neuregulin binding avtivity (Carraway et al. 1994). Heterodimerization of ErbB3 with other EGFR family members leads to cell proliferation and/or differentiation (Carraway and Cantley 1994).

        Amplification of this gene and/or over expression of its protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. In breast cancer it has been shown to dimerize with ErbB2 to drive tumour cell proliferation (Alimandi et al. 1995).

        Goat anti Human ErbB3 antibody recognizes human ErbB3 as a 185 kDa band in a number of human cell line lysates tested. It also demonstrates cross reacts with rodent cell lines.
      • Target Species
        Human
      • Western Blotting
        Anti ErbB3 antibody detects a band of approximately 185 kDa in MCF7 cell lysates.
        Anti ErbB3 antibody detects a band of approximately 185 kDa in MCF7 cell lysates.
        Anti ErbB3 antibody detects a band of approximately 185 kDa in MCF7 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
        Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide sequence C-HSRLFPKANAQRT from the C-terminal region ErbB3 isoform 1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg MCF7 lysate lyophilized in RIPA buffer.
        400μg MCF7 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        positive regulation of phosphatidylinositol 3-kinase cascade
        cranial nerve development
        neuron apoptosis
        integral to plasma membrane
        protein heterodimerization activity
        extracellular space
        Schwann cell differentiation
        ATP binding
        negative regulation of signal transduction
        transmembrane receptor protein tyrosine kinase activity
        phosphatidylinositol 3-kinase cascade
        protein phosphorylation
        regulation of cell proliferation
        negative regulation of neuron apoptosis
        wound healing
        receptor complex
        protein homodimerization activity
        receptor signaling protein tyrosine kinase activity
        protein tyrosine kinase activator activity
        negative regulation of cell adhesion
        negative regulation of secretion
        growth factor binding
        heart development
        basolateral plasma membrane
        transmembrane receptor protein tyrosine kinase signaling pathway
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (H/L):HRP5160-25042 mgC, E, WB
        5160-2504
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (H/L):HRP5160-25042 mgC, E, WB
        5160-2504
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Goat IgG (H/L):HRP5160-25042 mgC, E, WB
        5160-2504

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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