ErbB2 Antibody | 4D5-8

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ErbB2 Antibody | 4D5-8 gallery image 1

Purity assessment by SDS-PAGE

0.5 μg of Human Anti-ErbB2 Antibody, MCA6092 (trastuzumab biosimilar, research grade) and trastuzumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.

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Monodispersity assessment HPLC-SEC

Size exclusion chromatography (SEC) of Human Anti-ErbB2 Antibody, MCA6092 (trastuzumab biosimilar, research grade) and trastuzumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl; flow rate: 200 μl/min; inj. volume [μl]: 2; temp.: 25 °C; detection: 280 nm; sample: mAb (0.5-5 mg/ml)

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Trastuzumab bridging ELISA for pharmacokinetic (PK) assay development

A microtiter plate was coated overnight with Human Anti-Trastuzumab Antibody (clone AbD18018, HCA168) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab (therapeutic reference product) or Human Anti-ErbB2 Antibody, MCA6092 (trastuzumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody (clone AbD18018_hIgG1, HCA177) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®.

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  • Human anti ErbB2 (Trastuzumab Biosimilar)
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-ErbB2 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug trastuzumab (Herceptin) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.
    • Product Type
      Monoclonal Antibody
    • Clone
      4D5-8
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA6092Edatasheet pdfdatasheet pdf0.2 mg
      MCA6092
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human Anti-ErbB2 Antibody is a research grade biosimilar of the monoclonal antibody drug trastuzumab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic trastuzumab antibody clone 4D5-8.

        This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-trastuzumab antibodies, for example HCA168 and HCA167. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-trastuzumab antibody in IgG1 format as a reference standard, for example HCA176 or HCA177.

        Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

        This product is NOT FOR THERAPEUTIC USE.

        View a summary of supporting anti-trastuzumab antibody products.
      • Intended Use
      • Target Species
        Human
      • Product Form
        Human IgG1 (kappa) antibody - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.02% Proclin 300
      • Immunogen
        A431 cells (human epidermoid carcinoma) (over)expressing EGFR
      • Purity
        >98% by SDS PAGE
      • Approx. Protein Concentrations
        IgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
      • Licensed Use
        For in vitro research purposes only. Research grade biosmilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional ErbB2 Antibody Formats

      Formats Clone Applications Sizes available
      ErbB2 Antibody : Purified 4D5-8 E 0.2 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Trastuzumab:HRPHCA176P0.1 mgE
            HCA176P
            Human anti Trastuzumab:HRPHCA177P0.1 mgE
            HCA177P
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            Human anti TrastuzumabHCA1660.1 mgE
            HCA166
            Human anti TrastuzumabHCA1670.1 mgE
            HCA167
            Human anti TrastuzumabHCA1680.1 mgE
            HCA168
            Human anti TrastuzumabHCA1690.1 mgE
            HCA169
            Human anti TrastuzumabHCA1760.1 mgE
            HCA176
            Human anti TrastuzumabHCA1770.1 mgE
            HCA177
            Human anti Trastuzumab (Drug/Target Complex)HCA2630.1 mgE
            HCA263
            Human anti TrastuzumabHCA2700.1 mgE
            HCA270
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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