Ephrin B2

//www.bio-rad-antibodies.com/human-ephrin-b2-recombinant-protein-php233.html?RenderPageSpecific=1
Ephrin B2 gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • Ephrin B2 thumbnail image 1
  • Recombinant Human Ephrin B2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP233E, WBdatasheet pdfdatasheet pdf25 µg
    PHP233
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Ephrin B2 (EFNB2) is a type I transmembrane protein and member of the ephrin family, which acts as a ligand for the receptor tyrosine kinases EPHB4 and EPHB3 and has been linked with a range of physiological systems including neurotransmission, synaptic plasticity, lymphatic and renal development, tumour metastasis and bone remodelling. Ligation of EFNB2 with complementary EphB receptors on adjacent cells results in a combination of forward (EphB receptors) and reverse (ephrin B2) signalling, which is central to tissue development and remodelling functions.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised.
    • Reconstitution
      Reconstitute with 50 ul distilled water or sterile PBS
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant 6xHis-tagged EFNB2-Fc expressed in HEK293E cells
    • Preservative Stabilisers
      None present
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml after reconstitution.
    • Molecular Weight
      52.8kD (472 amino acid residues with tag)
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -70oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      ephrin receptor binding
      cell differentiation
      integral to plasma membrane
      cell-cell signaling
      nervous system development
      interspecies interaction between organisms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ephrin B2 Formats

    Formats Applications Sizes available
    Ephrin B2 : Rec. Protein E, WB 25 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

               
              No experiment is complete without all the pieces

              You may also be interested in...