Ephrin B2 (EFNB2) is a type I transmembrane protein and member of the ephrin family, which acts as a ligand for the receptor tyrosine kinases EPHB4 and EPHB3 and has been linked with a range of physiological systems including neurotransmission, synaptic plasticity, lymphatic and renal development, tumour metastasis and bone remodelling. Ligation of EFNB2 with complementary EphB receptors on adjacent cells results in a combination of forward (EphB receptors) and reverse (ephrin B2) signalling, which is central to tissue development and remodelling functions.
Purified recombinant protein - lyophilised.
Reconstitute with 50 ul distilled water or sterile PBS Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant 6xHis-tagged EFNB2-Fc expressed in HEK293E cells
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml after reconstitution.
52.8kD (472 amino acid residues with tag)
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
His-tag is a registered trademark of EMD Biosciences.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.