Ephrin B2

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  • Recombinant Human Ephrin B2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP233E, WBdatasheet pdfdatasheet pdf25 µg
    PHP233
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Ephrin B2 (EFNB2) is a type I transmembrane protein and member of the ephrin family, which acts as a ligand for the receptor tyrosine kinases EPHB4 and EPHB3 and has been linked with a range of physiological systems including neurotransmission, synaptic plasticity, lymphatic and renal development, tumour metastasis and bone remodelling. Ligation of EFNB2 with complementary EphB receptors on adjacent cells results in a combination of forward (EphB receptors) and reverse (ephrin B2) signalling, which is central to tissue development and remodelling functions.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised.
    • Reconstitution
      Reconstitute with 50 ul distilled water or sterile PBS
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant 6xHis-tagged EFNB2-Fc expressed in HEK293E cells
    • Preservative Stabilisers
      None present
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml after reconstitution.
    • Molecular Weight
      52.8kD (472 amino acid residues with tag)
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -70oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      ephrin receptor binding
      cell differentiation
      integral to plasma membrane
      cell-cell signaling
      nervous system development
      interspecies interaction between organisms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ephrin B2 Formats

    Formats Applications Sizes available
    Ephrin B2 : Rec. Protein E, WB 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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