EPHB4 Antibody | AbD01327
HuCAL Fab, bivalent, myc-his6-tagged
Enlarge- Human anti EPHB4
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Product TypeMonoclonal Antibody
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CloneAbD01327
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IsotypeHuCAL Fab bivalent
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
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Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Human anti EPHB4, clone AbD01327 recognizes the receptor tyrosine kinase EPHB4, a member of the largest known family of these kinases, and plays an important role in a variety of processes during embryonic development including pattern formation, cell aggregation and migration, segmentation, neural development, angiogenesis, and vascular network assembly.
EPHB4, along with its ligand, Ephrin-B2, is essential for vascular remodeling, maturation and directed growth. Both Ephrin-B2 and EPHB4 targeted gene knock-outs in mice show lack of remodeling of the primitive vascular plexus to form mature vascular structures. EPHB4 expression is restricted to venous endothelial cells. EPHB4 and Ephrin-B2 are also essential for defining the boundaries between arterial and venous domains, which persist in the adult. EPHB4 selectively binds Ephrin-B2 and not other Ephrin B ligands.
The B class Ephrins are comprised of transmembrane proteins with an intracellular domain that can elaborate reverse signaling. Ligand-receptor binding leads to protein clustering followed by receptor activation. Furthermore, interaction between EPHB4 and Ephrin-B2 can provoke bidirectional signaling. It has been shown in vitro that EPHB4-mediated forward signaling restricts intermingling of cells and supports cellular segregation, whereas reverse signaling from Ephrin-B2 stimulates migration and sprouting angiogenesis. Downstream signaling by EPHBs regulates cell proliferation, cytoskeletal organization, and migration.
EPHB4 is abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in foetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
In Western blotting, clone AbD01327 may cross-react with EPHB1, however in immunohistology the staining pattern is consistent with specificity only for EPHB4.
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Intended Use
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Target SpeciesHuman
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Species Cross-Reactivity
N.B. Antibody reactivity and working conditions may vary between species.Target Species Cross Reactivity Mouse Expected from Sequence -
Product FormA bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain. This antibody is supplied lyophilised.
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ReconstitutionReconstitute with 1.0ml distilled water
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PreparationMetal chelate affinity chromatography
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin -
ImmunogenRecombinant EPHB4 fusion protein (Leu563 - Tyr987), corresponding to the intracellular part of the protein, including the kinase domain and an SAM (sterile alpha motif) domain.
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Purity
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Approx. Protein Concentrations0.1 mg/ml after reconstitution
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered saline
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StoragePrior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life6 months from date of reconstitution.
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GO Termsregulation of angiogenesisintegral to plasma membranecell surfaceprotein phosphorylationtransmembrane receptor protein tyrosine kinase signaling pathwaycell proliferationorgan morphogenesisephrin receptor activityATP binding
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UniProt
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Entrez Gene
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AcknowledgementsSold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. -
Licensed UseFor in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA 2ug/ml Immunohistology - Paraffin 10ug/ml Western Blotting 2ug/ml - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical AdviceRecommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Recommended Protocol
- ELISADue to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional EPHB4 Antibody Formats
| Formats | Clone | Applications | Sizes available |
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| EPHB4 Antibody : Purified | AbD01327 | E, P, WB | 0.1 mg |
- Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:Alk. Phos. | 0500-0100 | 0.5 ml | E, WB | 0500-0100 | |
| Goat anti Human IgG F(ab')2:Alk. Phos | STAR126A | 0.5 ml | E, WB | STAR126A | |
| Goat anti Human IgG F(ab')2:Biotin | STAR126B | 0.5 mg | C, E, F, WB | STAR126B | |
| Mouse anti c-Myc:HRP | MCA2200P | 0.1 mg | C, E, P, WB* | MCA2200P | |
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | 0.5 ml | E, WB | 0500-0099 | |
| Goat anti Human IgG F(ab')2:HRP | STAR126P | 0.5 ml | E, WB | STAR126P | |
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | 125 µl | E, WB | MCA5995P |
Recommended Negative Isotype Control
Useful Reagents
Recommended Positive Controls
Histology Controls
References
1. Xia, G. et al. (2005) EphB4 expression and biological significance in prostate cancer.Cancer Res. 65: 4623-4632.
