Endothelial Cells Antibody | KG7/30

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  • Mouse anti Human Endothelial Cells
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  • Product Type
    Monoclonal Antibody
  • Clone
    KG7/30
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4108-2004C *datasheet pdfdatasheet pdf0.5 ml
    4108-2004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • 4108-2004 is an interesting marker for the characterisation of healthy tissues. When used in combination with other monoclonal antibodies it enables the characterisation of tumorous tissue. Expression of the KG7/30 antigen decreases with increasing malignancy of endothelium-derived tumour cells.

      This antibody stains endothelial cells in healthy tissues of all organs, apart from small capillaries. The antigen expression is more pronounced in venous than in arterial vessel types and decreases in tumourous tissues.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Use 0.5 ml of sterile distilled water. The resulting solution will contain 0.1 mg/ml IgG.
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.01%Thiomersal
      1%Bovine Serum Albumin
    • Immunogen
      Cultured human endothelial cells
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)1/501/200
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human placenta or tonsil
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Endothelial Cells Antibody Formats

    Formats Clone Applications Sizes available
    Endothelial Cells Antibody : Purified KG7/30 C * 0.5 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human placenta or tonsil

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