Endothelial Cells Antibody | 1F10

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  • Rat anti Human Endothelial Cells (Fully Differentiated)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1F10
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4108-2108C *datasheet pdfdatasheet pdf0.1 mg
    4108-2108
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Human Endothelial cells antibody, clone 1F10 recognises a 150 kDa antigen present on fully differentiated continuous blood endothelial cells. Expression is low and inconsistent on sinusoidal endothelial cells of the liver and dermal lymphatic endothelia. Expression is absent on splenic sinusoidal endothelial cells (Goerdt et al. 1989).

      Immunohistochemical studies indicate that the antigen recognized by Rat anti Human Endothelial cells antibody, clone 1F10 is strongly up-regulated in the muscularis propria of patients with Chron's disease, suggesting a possible role in the maintainence of chronic inflammation in this condition (Lügering et al. 1995).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      With 0.5 ml sterile distilled water
    • Preparation
    • Preservative Stabilisers
      0.01% Thiomersal, 1% Bovine Serum Albumin.
    • Immunogen
      Cultured HUVECs.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      12 months from date of reconstitution.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)1/501/200
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human placenta
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Endothelial Cells Antibody Formats

    Formats Clone Applications Sizes available
    Endothelial Cells Antibody : Purified 1F10 C * 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human placenta

              References

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