Endonuclease G Antibody

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Endonuclease G Antibody gallery image 1

Staining of formalin fixed, paraffin processed human pancreas with Rabbit anti Human EndoG at 15 µg/ml. (AHP730)

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Endonuclease G Antibody gallery image 2

Western blot analysis of whole cell lysates from mouse 3T3 (M) and Human HepG2 (H) cells probed with Rabbit anti endonuclease G (AHP730)

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Endonuclease G Antibody gallery image 3

Published customer image:
Rabbit anti endonuclease G antibody (AHP730) used for the evaluation of Endo G expression in rat mitochondria by western blotting.
Image caption:
Release of mitochondrial intermembrane space proteins.
Mitochondria were incubated as described in the legend of fig. 1, whereupon they were sedimented by centrifugation and proteins of the resulting pellets (P) and supernatants (S) were analyzed by immunoblotting. Panel A: release of cytochrome c, Smac/DIABLO, Omi/HtrA2, AIF, endonuclease G, and LACTB by histones, tBID and p53. The concentrations used were: 5 μM histone H1.2; 10 μM histone H2A, 10 μM histone H2B, 5 μM histone H3, 5 μM histone H4, 1 μM tBID, and 1 μM p53. Panel B: effect of cyclosporin A on the histone-induced release of cytochrome c and Smac/DIABLO. Panel C: accessibility of AIF and LACTB to trypsin hydrolysis in the presence of histones.

From: Cascone A, Bruelle C, Lindholm D, Bernardi P, Eriksson O (2012)
Destabilization of the Outer and Inner Mitochondrial Membranes by Core and Linker Histones.
PLoS ONE 7(4): e35357.

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Endonuclease G Antibody gallery image 4

Published customer image:
Rabbit anti endonuclease G antibody (AHP730) used for the evaluation of EndoG expression in HCC1187 cells treated with TG02 in vitro by western blotting.
Image caption:
TG02 triggers apoptosis through caspase-dependent and independent pathways
(A) HCC1187 cells were treated with TG02 (1 μM) for different times (0, 6, 12 and 24 hours). Loss of mitochondrial membrane potential and viability were analyzed by flow cytometry after staining with TMRE or propidium iodide (PI), respectively. (B) Expression of proteins involved in apoptotic mechanisms was analyzed by Western blot after treatment of HCC1187 cells with TG02 (1 μM) at the indicated times. GAPDH was used as loading control. (C) Release of proteins from the intermembrane space of mitochondria in HCC1187 cells treated for 12 and 24 hours with TG02 (1 μM) was analyzed by Western blot after subcellular fractionation. COX IV and ERK1/2 were analyzed as mitochondrial and cytosolic markers, respectively. (D) Role of caspases in TG02-induced cell death. Induction of cell death by TG02 was analyzed in HCC1187 cells by flow cytometry after 1 hour of pretreatment with the pan-caspase inhibitor Z-VAD-FMK (25 μM), followed by treatment with TG02 (1 μM) for 24 hours.

From: Ortiz-Ruiz MJ, Álvarez-Fernández S, Parrott T, Zaknoen S, Burrows FJ, Ocaña A, Pandiella A, Esparís-Ogando A.
Therapeutic potential of ERK5 targeting in triple negative breast cancer.
Oncotarget. 2014 Nov 30;5(22):11308-18.

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  • Rabbit anti Endonuclease G
  • Rabbit anti Endonuclease G
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP730P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP730
    AHP730TP *, WBdatasheet pdfdatasheet pdf50 µg
    AHP730T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Endonuclease G antibody recognizes human endonuclease G, also known as endoG. Endo G is a 297 amino acid, including a 48 amino acid transit peptide ~36 kDa mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis.

      EndoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria (Li et al. 2001).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      MouseExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to endonuclease G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      Pack Size: 50 µg
      0.09%Sodium Azide (NaN3)
      Pack Size: 0.1 mg
      0.02%Sodium Azide (NaN3)
      Pack Size: 50 µg
      0.09%Sodium Azide (NaN3)
      Pack Size: 0.1 mg
      0.02%Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 50 µgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mgIgG concentration 1.0 mg/ml
      Pack Size: 50 µgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mgIgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      metal ion binding
      endonuclease activity
      nucleic acid binding
      mitochondrion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1ug/ml2ug/ml
      Immunohistology - Paraffin(1)15ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1ug/ml2ug/ml
      Immunohistology - Paraffin(1)15ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human pancreas
    • Histology Positive Control Tissue
      Human pancreas
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AHP730 detects a band of approximately 35kDa in HepG2 and 3T3 cell lysates.
    • Western Blotting
      AHP730 detects a band of approximately 35kDa in HepG2 and 3T3 cell lysates.
    • Instructions For Use
    • Instructions For Use

    Additional Endonuclease G Antibody Formats

    Formats Applications Sizes available
    Endonuclease G Antibody : Purified P *, WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human pancreas
              Human pancreas
              • Application NameReference Images
                Western Blotting

              References

              Further Reading

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