Chicken anti Human Elongin C antibody recognizes human Elongin C (EloC), otherwise known as transcription elongation factor B polypeptide 1, a general transcription elongation factor, which stimulates RNA polymerase II transcription elongation beyond template-encoded arresting sites.
Elongin exists as a heterotrimer consisting of an A (A1, A2 or A3), B and C subunit. Subunit A is transcriptionally active, with activity strongly enhanced by binding to the dimeric complex of the elongin regulatory subunits B and C (elongin BC complex), which is mediated by subunit C.
Purified IgY fraction - liquid
Antiserum to human Elongin C was raised by repeated immunisation of chickens with highly purified antigen.
0.09% Sodium Azide (NaN3)
Human GST-Elongin C fusion protein.
>98% IgY purity with no contaminants detected by SDS-PAGE, IEP or RID.
Approx. Protein Concentrations
IgY concentration 17.0mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.