ELF3 Antibody (PrecisionAbTM Antibody)

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ELF3 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with ELF3 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to ELF3 (molecular weight 47 kDa).

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  • Rabbit anti ELF3
  • ELF3 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    ELF3 antibody recognizes the ETS-related transcription factor Elf-3, also known as E74-like factor 3 (ets domain transcription factor), epithelial-restricted with serine box, epithelium-restricted Ets protein ESX or epithelium-specific Ets transcription factor 1.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00753WBdatasheet pdfdatasheet pdf100 µl
      VPA00753
      VPA00753KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00753KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human ELF3 antibody recognizes the ETS-related transcription factor Elf-3, also known as E74-like factor 3 (ets domain transcription factor), epithelial-restricted with serine box, epithelium-restricted Ets protein ESX or epithelium-specific Ets transcription factor 1.

        Rabbit anti Human ELF3 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti ELF3 antibody recognizes a band of approximately 47 kDa in A431 cell lysates
        Anti ELF3 antibody recognizes a band of approximately 47 kDa in A431 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody purified by affinity chromatography on immunogen
        20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.01% Thiomersal
        1% Bovine Serum Albumin
        <20% Glycerol
        0.01% Thiomersal
        1% Bovine Serum Albumin
        <20% Glycerol
      • Immunogen
        Synthetic peptide encompassing part of the C terminal region of human ELF3
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg A431 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        mammary gland involution
        sequence-specific DNA binding
        transcription activator activity
        Golgi apparatus
        epithelial cell differentiation
        sequence-specific DNA binding transcription factor activity
        epidermis development
        protein binding
        transcription repressor activity
        inflammatory response
        transcription from RNA polymerase II promoter
        transcription coactivator activity
        nucleus
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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