EIF3A Antibody (PrecisionAbTM Antibody)

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EIF3A Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with EIF3A antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to EIF3A (molecular weight 166 kDa)

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  • EIF3A Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti EIF3A
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    EIF3A antibody recognizes eukaryotic translation initiation factor 3 subunit A, also known as EIF3, p180 subunit, centrosomin homolog, cytoplasmic protein p167, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185, eukaryotic translation initiation factor 3 subunit 10 or eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD). This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00215WBdatasheet pdfdatasheet pdf100 µl
      VPA00215
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human EIF3A antibody recognizes the eukaryotic translation initiation factor 3 subunit A, also known as EIF3, p180 subunit, centrosomin homolog, cytoplasmic protein p167, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185 and eukaryotic translation initiation factor 3 subunit 10.

        Rabbit anti Human Eukaryotic translation initiation factor 3 subunit A antibody detects a band of 166 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti EIF3A detects a band of approximately 166 kDa in PC-12 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH conjugated synthetic peptide between 1219-1247 amino acids from the C-terminal region of human EIF3A
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        structural molecule activity
        cytosol
        translation initiation factor activity
        eukaryotic translation initiation factor 3 complex
        protein binding
        formation of translation initiation complex
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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