EGF R (pTyr1068) Antibody

EGF R (pTyr1068) Antibody gallery image 1

Western blot analysis of extracts from A431 cells untreated or treated with EGF using Rabbit anti EGF R (pTyr1068) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes

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  • Rabbit anti EGF R (pTyr1068)
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    • Product Type
      Polyclonal Antibody
    • Isotype
      Polyclonal IgG
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      AHP2590WBdatasheet pdfdatasheet pdf50 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Rabbit anti EGF R (pTyr1068) antibody recognizes the epidermal growth factor receptor (EGF R), also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at tyrosine 1068. EGF R is a transmembrane glycoprotein and a member of the ErbB receptor tyrosine kinase family. EGF R is a cell surface receptor for members of the epidermal growth factor family.

        EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006).

        EFG R is autophosphorylated at tyrosine 1068 upon ligand binding and receptor dimerization.
      • Intended Use
      • Target Species
      • Product Form
        Purified IgG - liquid
      • Reconstitution
      • Preparation
      • Antiserum Preparation
        Antiserum to EGF R (pTyr1068) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
      • Preservative Stabilisers
        0.02% Sodium Azide
        50% Glycerol
      • Immunogen
        Phospho specific-peptide corresponding to residues surrounding tyrosine 1068 of human EGF R
      • Purity
      • Approx. Protein Concentrations
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at -20oC
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • UniProt
      • Entrez Gene
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/5001/1000

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
        Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
      • Instructions For Use

      Additional EGF R (pTyr1068) Antibody Formats

      Formats Applications Sizes available
      EGF R (pTyr1068) Antibody : Purified WB 50 µl
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

            Recommended Positive Controls

              Histology Controls

                Further Reading

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