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EBP50 Antibody | EBP-10

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  • Mouse anti Human EBP50
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2423IP, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human EPB50 antibody, clone EPB-10 recognizes ezrin/radixin/moesin (ERM)-binding phosphoprotein of 50kDa (EBP50) otherwise known as Na+/H+ exchanger regulatory factor 1 (NHERF1), Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1 or Solute carrier family 9 isoform A3 regulatory factor 1. EBP50 is a 358 amino acid ~50kDa cytoplasmic adaptor molecule containing 2 PDZ domains. EBP50 is expressed in tissues containing polarized epithelia, including the small intestine, placenta, liver and kidney.

      The ERM family of membrane-cytoskeletal linking proteins act to maintain a regulated linkage between actin filaments (F-actin) and the plasma membrane. Studies have revealed that EBP50 is an important ERM binding partner, which interacts with the N-terminal FERM (four point one ERM) domain of ERM proteins, via its C-terminal domain. EBP50 colocalizes with actin and ezrin, in the apical mircovilli of epithelial cells and this role as binding partner has been further shown through the interaction of EBP50 with the transmembrane adaptor protein known as Protein Associated with Glycosphingolipid-enriched microdomains (PAG), in T cells (Reczek et al. 1997). Mutations in the gene encoding EBP50 lead to the disease Nephrolithiasis/osteoporosis, hypophosphatemic, 2 characterized by impairedphosphate metabolism (Courbebaisse et al. 2012). Other ligands which bind to the PDZ domains of EBP50, include beta-catenin, cystic fibrosis transmembrane conductance regulator and the type II Na/Pi cotransporter Npt2, implicating EBP50 in a diverse range of biological systems.

      Mouse anti Human EPB50 antibody, clone EPB-10 originally raised by Brdičková et al. has been sucessfully used in western blotting and immunofluorescence studies of EBP expression and localization.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2423 detects a band of approximately 50 kDa in Breast Carcinoma & Jurkat cell lysates.
    • Instructions For Use

    Additional EBP50 Antibody Formats

    Formats Clone Applications Sizes available
    EBP50 Antibody : Purified EBP-10 IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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