E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) Antibody

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E3 UBIQUITIN-PROTEIN Ligase  CBL (pTyr700) Antibody gallery image 1

Western blot analysis of extracts from HT-29 cells treated or untreated with UV ( for 15-30 minutes) using Rabbit anti E3 ubiqutin-protein ligase CBL (pTyr700) antibody

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E3 UBIQUITIN-PROTEIN Ligase  CBL (pTyr700) Antibody gallery image 2

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti E3 ubiqutin-protein ligase CBL (pTyr700) antibody

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  • Rabbit anti E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2585P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2585
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti E3 ubiquitin-protein ligase CBL (pTyr700) antibody recognizes the Casitas B-lineage lymphoma (CBL) E3 ubiquitin ligase, also known as c-CBL proto-oncogene and ring finger protein 55, when phosphorylated at tyrosine 700. The CBL protein is a RING finger E3-ubiquitin ligase that plays a key role in signal transduction through mediating monoubiquitination and endocytosis of receptor tyrosine kinases (Haglund et al. 2002, Dikic and Giordano 2003).

      E3 ubiqutin-protein ligase CBL is phosphorylated at tyrosine 700 by Fyn, Yes and Syk (Feshchenko et al. 1998).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to E3 ubiqutin-protein ligase CBL (pTyr700) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Phospho specific-peptide corresponding to residues surrounding tyrosine 700 of human E3 ubiqutin-protein ligase CBL
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      zinc ion binding
      sequence-specific DNA binding transcription factor activity
      SH3 domain binding
      cytosol
      negative regulation of epidermal growth factor receptor signaling pathway
      ubiquitin-protein ligase activity
      nucleus
      signal transducer activity
      epidermal growth factor receptor signaling pathway
      positive regulation of receptor-mediated endocytosis
      calcium ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/100
      Western Blotting1/5001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) Antibody Formats

    Formats Applications Sizes available
    E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

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          Recommended Positive Controls

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              Further Reading

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