E3 UBIQUITIN-PROTEIN Ligase CBL Antibody

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E3 UBIQUITIN-PROTEIN Ligase CBL Antibody gallery image 1

Western blot analysis of (A) Jurkat and (B) K562 whole cell lysate probed with Rabbit anti E3 ubiquitin-protein ligase CBL

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  • Rabbit anti E3 UBIQUITIN-PROTEIN Ligase CBL
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2416WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2416
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti E3 ubiquitin-protein ligase CBL antibody recognizes the Casitas B-lineage lymphoma (CBL) E3 ubiquitin ligase, also known as c-CBL proto-oncogene and ring finger protein 55. As the name implies, the viral form of CBL, v-CBL, has been shown to induce lymphoma and leukemia in mice (de Jong et al. 1995).

      The CBL protein is a RING finger E3-ubiquitin ligase that plays a key role in signal transduction processes through mediating monoubiquitination and thereby endocytosis of receptor tyrosine kinases (Haglund et al. 2002, Dikic and Giordano 2003). In addition to this role, CBL has also been reported to act as an adaptor protein as well as a mediator of cell signaling processes by changing the cellular localization of substrates (Liyasova et al. 2015).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Antiserum Preparation
      Antiserum to E3 ubiquitin-protein ligase CBL was raised by repeated immunisation of rabbits with highly purified antigen
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.2% Gelatin
    • Immunogen
      Peptide mapping to the carboxy terminal domain of human E3 ubiquitin-protein ligase CBL
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      cytosol
      nucleus
      epidermal growth factor receptor signaling pathway
      ubiquitin-protein ligase activity
      positive regulation of receptor-mediated endocytosis
      zinc ion binding
      SH3 domain binding
      negative regulation of epidermal growth factor receptor signaling pathway
      calcium ion binding
      sequence-specific DNA binding transcription factor activity
      signal transducer activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti E3 ubiquitin-protein ligase CBL recogninizes a band of approximately117 kDa when run under reducing conditions
    • Instructions For Use

    Additional E3 UBIQUITIN-PROTEIN Ligase CBL Antibody Formats

    Formats Applications Sizes available
    E3 UBIQUITIN-PROTEIN Ligase CBL Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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