DPM1 Antibody

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Human liver cell lysate probed with Goat anti Human DPM1 N-Terminal (AHP2108)

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  • Goat anti Human DPM1 (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2108E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2108
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • AHP2108 specifically recognises an epitope within the N-terminal (NT) region of human DPM1, otherwise known as dolichol-phosphate mannosyltransferase, a member of the glycosyltransferase 2 family, expressed in the endoplasmic reticulum (ER).

      DPM1 is involved in the formation of dolichol phosphate mannose (Dol-P-Man), through the transfer of mannose from GDP-mannose to dolichol monophosphate. Dol-P-Man then acts as a mannosyl donor for protein N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation. Defects in DPM1 are responsible for the congenital disorder known as glycosylation type 1E (CDG1E), a metabolic deficiency in glycoprotein biosynthesis, resulting in severe mental and psychomotor retardation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human DPM1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence RELEVRSPRQNKYS-C from the N-Terminal region of DPM1 (NP_003850.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      dolichol-phosphate-mannose synthase complex
      dolichol metabolic process
      endoplasmic reticulum membrane
      membrane fraction
      GPI anchor biosynthetic process
      dolichol-linked oligosaccharide biosynthetic process
      dolichyl-phosphate-mannose-protein mannosyltransferase activity
      protein O-linked mannosylation
      protein N-linked glycosylation via asparagine
      C-terminal protein lipidation
      dolichyl-phosphate beta-D-mannosyltransferase activity
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/32000
      Western Blotting0.10.3ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP2108 detects a band of approximately 28kDa in human liver cell lysates.
    • Instructions For Use

    Additional DPM1 Antibody Formats

    Formats Applications Sizes available
    DPM1 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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