Dopamine Beta Hydroxylase Antibody

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Dopamine Beta Hydroxylase Antibody gallery image 1

Western blot of 2μg of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa DBH protein

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  • Sheep anti Human Dopamine Beta Hydroxylase (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP979WBdatasheet pdfdatasheet pdf0.1 ml
    AHP979
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Human Dopamine beta hydroxylase recognizes an epitope within the C-terminal region (CT) of Dopamine Beta-Hydroxylase (DBH). DBH is a member of the copper type II, ascorbate-dependent monooxygenase family, which catalyzes the conversion of dopamine to norepinephrine, a key neurotransmitter in the central and peripheral nervous systems.

      The effects of DBH plasma levels have been a focus area for both neurological and psychiatric disorders, including Parkinson’s disease, hypertension, depression, schizophrenia, drug abuse and stress (Cubells and Zabetian 2004).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human Dopamine Beta-Hydroxylase (CT) were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      Synthetic peptide corresponding to the C-terminal region of human Dopamine Beta-Hydroxylase, conjugated to KLH.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM Hepes pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      dopamine beta-monooxygenase activity
      transport vesicle membrane
      soluble fraction
      membrane fraction
      extracellular region
      oxidation-reduction process
      hormone biosynthetic process
      chromaffin granule lumen
      chromaffin granule membrane
      L-ascorbic acid binding
      integral to membrane
      histidine catabolic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP979 detects a band of approximately 75kDa in human adrenal medulla cell lysates.
    • Instructions For Use

    Additional Dopamine Beta Hydroxylase Antibody Formats

    Formats Applications Sizes available
    Dopamine Beta Hydroxylase Antibody : Purified WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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