DOK2 (pTyr299) Antibody

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DOK2 (pTyr299) Antibody gallery image 1

Western blot analysis of extracts from Jurkat cells untreated or treated with Insulin using Rabbit anti DOK2 (pTyr299) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Insulin (100nM) for 10 minutes

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DOK2 (pTyr299) Antibody gallery image 2

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti DOK2 (pTyr299) antibody

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  • DOK2 (pTyr299) Antibody thumbnail image 1
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  • Rabbit anti DOK2 (pTyr299)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2584P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2584
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti DOK2 (pTyr299) antibody recognizes docking protein 2 (DOK2), also known as dok-2 and downstream of tyrosine kinase 2, when phosphorylated at tyrosine 299. DOK2 belongs to the downstream of tyrosine kinase (Dok) family of adaptor proteins involved in receptor signaling.

      DOK2 is phosphorylated by Bcr-Abl, receptor tyrosine kinases and also cytoplasmIc protein tyrosine kinases (Gérard et al. 2004).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to DOK2 (pTyr299) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Phospho specific-peptide corresponding to residues surrounding tyrosine 299 of human DOK2
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      blood coagulation
      insulin receptor binding
      identical protein binding
      cytosol
      leukocyte migration
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/100
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional DOK2 (pTyr299) Antibody Formats

    Formats Applications Sizes available
    DOK2 (pTyr299) Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Further Reading

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