DNA PKcs Antibody (PrecisionAbTM Antibody)

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DNA PKcs Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with DNA PKcs antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc™ MP with 41 second exposure. Arrow points to DNA PKcs (molecular weight >350 kDa)

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  • DNA PKcs Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti DNA PKcs
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    Mouse anti human DNA PKcs antibody recognizes the DNA-dependent protein kinase catalytic subunit, also known as PRKDC, DNA-PK catalytic subunit or hyper-radiosensitivity of murine scid mutation, complementing 1, p460. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00423WBdatasheet pdfdatasheet pdf100 µl
      VMA00423
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human DNA PKcs antibody recognizes DNA-dependent protein kinase catalytic subunit (DNA PKcs), also known as PRKDC and DNA-PK catalytic subunit.

        Encoded by the PRKDC gene, DNA PKcs functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. DNA PKcs is a member of the PI3/PI4-kinase family (provided by RefSeq, Jul 2010).

        Mouse anti Human DNA PKcs antibody detects a band of >350 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti DNA PKcs detects a band of approximately >350 kDa in MOLT-4 cell lysates
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
      • Immunogen
        E.coli-derived recombinant protein corresponding to aa 3844-4127 of human DNA PKcs
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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