DFF-45 Antibody

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DFF-45 Antibody gallery image 1

Western blot analysis of whole cell lysates from HeLa (H), Jurkat (J), A431 (A) and K562 (K) cell lines probed with Rabbit anti Human DFF45 (AHP433)

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DFF-45 Antibody gallery image 2

Immunocytochemical staining of HeLa cells with Rabbit anti Human DFF45 (AHP433)

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  • DFF-45 Antibody thumbnail image 1
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  • Rabbit anti Human DFF-45 (C-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP433C, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP433
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • AHP433 recognises the 45kD chain of a heterodimeric apoptosis related protein designated DFF (DNA fragmentation factor). DFF-45 is a substrate for Caspase-3, which cleaves the protein at two sites to generate factors that cause DNA fragmentation.

      AHP433 recognises both intact DFF-45 and cleaved fragments at the C-terminus.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to DFF-45 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Peptide from C-terminus of human DFF-45
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      negative regulation of apoptosis
      DNA fragmentation involved in apoptotic nuclear change
      plasma membrane
      identical protein binding
      mitochondrion
      cytosol
      deoxyribonuclease activity
      intracellular signal transduction
      nucleoplasm
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting1/10001/2000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional DFF-45 Antibody Formats

    Formats Applications Sizes available
    DFF-45 Antibody : Purified C, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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