Desmoglein 3 Antibody | 5G11

//www.bio-rad-antibodies.com/human-desmoglein-3-antibody-5g11-mca2273.html
Desmoglein 3 Antibody | 5G11 gallery image 1

Published customer image:
Mouse anti Human desmoglein-3 antibody, clone 5G11 (MCA2273) used for the evaluation of desmoglein-3 expression on human cells by immunofluorescence.
Image caption:
Desmoglein-3 and flotillin-2 colocalise in human epidermis and human epithelial cell lines.
Human skin cryosections, HaCaT and MCF10A cells were immunostained for Dsg3 (red) and flotillin-2 (green). In human epidermis, flotillin-2 is expressed throughout the epidermal layer and colocalises with Dsg3 in keratinocytes of the stratum basale and suprabasale. In HaCaT keratinocytes, flotillin-2 is localised at the cell membrane and in intracellular vesicular structures. Dsg3 is localised at the plasma membrane where it colocalises with flotillin-2. In MCF10A mammary epithelial cells, flotillin-2 and Dsg3 show a more profound colocalisation at the sites of cell-cell contact. The cell lines were grown on glass coverslips for at least 3 days. Scale bar: 20μm.

From: Völlner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R.
Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
Sci Rep. 2016 Jun 27;6:28820.

Enlarge
Desmoglein 3 Antibody | 5G11 gallery image 2

Published customer image:
Mouse anti Human desmoglein-3 antibody, clone 5G11 (MCA2273) used for the evaluation of desmoglein-3 expression on HaCaT cells by immunofluorescence.
Image caption:
Loss of flotillins results in pemphigus vulgaris like localisation of Dsg3 in HaCaT keratinocytes.
(a) HaCaT cells depleted of flotillin-1 (F1-shRNA), flotillin-2 (F2-shRNA) or control shRNA cells (sh-control) were grown for three days on glass coverslips, fixed and stained with Dsg3 (red) and flotillin-2 (green) antibodies. Note that the signals for Dsg3 in flotillin knockdown cells in (a) have been adjusted to make the morphological deficiencies more clear. (b) HaCaT keratinocytes were grown for two days on glass coverslips and treated with 150?μg/ml of either control-IgG (healthy person) or IgG from PV patients for 20?h. Cells were fixed and immunostained for Dsg3 (red) and flotillin-2 (green). Scale bar: 20?μm. (c) Quantification of flotillin-2 localisation in control IgG vs. PV IgG treated cells. At least 8 images/condition (total of >200 cells/condition) originating from three independent experiments were quantified for plasma membrane associated vs. intracellular flotillin-2 staining. Statistical analysis was done by Student's t test (***p?<0.001).

From: Völlner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R.
Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
Sci Rep. 2016 Jun 27;6:28820.

Enlarge
Desmoglein 3 Antibody | 5G11 gallery image 3

Published customer image:
Mouse anti Human desmoglein-3 antibody, clone 5G11 (MCA2273) used for the evaluation of desmoglein-3 expression on HaCaT cells by western blotting.
Image caption:
Flotillin depletion results in diminished Dsg3 and E-cadherin protein levels in HaCaT cells.
(a) HaCaT cells with a stable flotillin knockdown or control cells were grown until confluent, and equal protein amounts were analysed by Western blot. (b–f) Western blot signals were quantified by scanning densitometry and normalised against GAPDH. Bars represent the mean?±?SD of five (b–e) or four (f) independent experiments. One-way ANOVA with Bonferroni's multiple comparison test. ***p <0.001.

From: Völlner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R.
Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
Sci Rep. 2016 Jun 27;6:28820.

Enlarge
Desmoglein 3 Antibody | 5G11 gallery image 4

Published customer image:
Mouse anti Human desmoglein-3 antibody, clone 5G11 (MCA2273) used for the evaluation of desmoglein-3 expression on HaCaT cells by western blotting.
Image caption:
Flotillin knockdown weakens the adhesion strength in HaCaT keratinocytes but does not affect the raft association or detergent solubility of Dsg1 and Dsg3.
(a) HaCaT cells depleted of flotillin-1 (F1-shRNA) and flotillin-2 (F2-shRNA) and control shRNA cells (sh-control) were dissociated from the plate with dispase and the monolayers were mechanically disrupted. Fragments were fixed, stained and imaged. (b) Relative fragment number from six independent experiments. Bars represent the mean?±?SD. One-way ANOVA with Bonferroni's multiple comparison test. *p?<0,05, **p?<0,01, ***p?<0.001. (c–e) Rafts were isolated from control shRNA (c) and flotillin-1 (d) or flotillin-2 (e) depleted HaCaT cells using detergent extraction and density gradient centrifugation. Nine fractions were collected from the top of the gradient (fraction 1?=?the lightest fraction) to the bottom and analysed by Western blot. (f) Sequential detergent extraction of HaCaT control and flotillin knockdown cells. Cells were initially extracted with a Triton X-100 buffer followed by solubilisation of the Triton X-100 insoluble proteins with an SDS/urea buffer. Equal volumes of extracted proteins were analysed by Western blot. Please note that the total volume of the insoluble pool is twice that of the soluble one. The lysates shown in the last three rows were extracted with a NP-40 containing lysis buffer to facilitate solubilisation.

From: Völlner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R.
Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
Sci Rep. 2016 Jun 27;6:28820.

Enlarge
Desmoglein 3 Antibody | 5G11 gallery image 5

Published customer image:
Mouse anti Human desmoglein-3 antibody, clone 5G11 (MCA2273) used for the evaluation of desmoglein-3 expression on HaCaT cells by western blotting and immunofluorescence.
Image caption:
Inhibition of lysosomal degradation or endocytosis restores Dsg3 level and improves desmosomal morphology in flotillin knockdown cells.
(a) Flotillin depleted or control HaCaT cells were treated for 24?h with BafA (50?nM) to block lysosomal degradation. Cell lysates were analysed by Western blot. (b) Western blot signals for Dsg3 (upper) and Dsg1 (lower) were quantified by scanning densitometry and normalised against GAPDH. Bars represent the mean?±?SD of 6 independent experiments. Two-way ANOVA. *p?<0,05, **p?<0,01, ***p?<0.001. (c) Flotillin knockdown or control cells were serum-starved overnight and treated for two hours with 80?μM Dynasore to inhibit dynamin-dependent endocytic uptake of Dsg3 from the plasma membrane. DMSO served as a control. Cells were fixed and immunostained for Dsg3. Scale bar: 20?μm.

From: Völlner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R.
Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
Sci Rep. 2016 Jun 27;6:28820.

Enlarge
Desmoglein 3 Antibody | 5G11 gallery image 6

Published customer image:
Mouse anti Human desmoglein-3 antibody, clone 5G11 (MCA2273) used for the evaluation of desmoglein-3 expression on human skin cryosections by immunofluorescence.
Image caption:
Desmoglein-3 and flotillin-1 colocalise in human epidermis.
Human skin cryosections were immunostained for Dsg3 (red) and flotillin-1 (green). In human epidermis, flotillin-1 is expressed throughout the epidermal layer and colocalises with Dsg3 in keratinocytes of the stratum basale and suprabasale. In HaCaT keratinocytes, flotillin-1 and flotillin-2 extensively colocalise at the cell membrane and in intracellular vesicular structures.

From: Völlner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R.
Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
Sci Rep. 2016 Jun 27;6:28820.

Enlarge
Desmoglein 3 Antibody | 5G11 gallery image 7

Published customer image:
Mouse anti Human desmoglein-3 antibody, clone 5G11 (MCA2273) used for the evaluation of desmoglein-3 expression on human skin cryosections by immunofluorescence.
Image caption:
Inhibition of lysosomal degradation or endocytosis doesnot change desmoplakin and ?-catenin levels but results in lysosomal accumulation of Dsg3 in flotillin knockdown cells.
Flotillin depleted or control HaCaT cells were treated for24 h with BafA (50 nM) to block lysosomal degradation. After BafA treatment, Dsg3 (red) accumulates in lysosomes and colocalises with the lysosomal marker LAMP1 (green). Scale bar: 20 μm

From: Völlner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R.
Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes.
Sci Rep. 2016 Jun 27;6:28820.

Enlarge
  • Desmoglein 3 Antibody | 5G11 thumbnail image 1
  • Desmoglein 3 Antibody | 5G11 thumbnail image 2
  • Desmoglein 3 Antibody | 5G11 thumbnail image 3
  • Desmoglein 3 Antibody | 5G11 thumbnail image 4
  • Desmoglein 3 Antibody | 5G11 thumbnail image 5
  • Desmoglein 3 Antibody | 5G11 thumbnail image 6
  • Desmoglein 3 Antibody | 5G11 thumbnail image 7
  • Mouse anti Human Desmoglein 3
  • Mouse anti Human Desmoglein 3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5G11
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2273C, IP, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2273
    MCA2273TC, IP, P *, WBdatasheet pdfdatasheet pdf25 µg
    MCA2273T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Desmoglein 3 antibody, clone 5G11 recognizes human desmoglein-3, also known as 130 kDa pemphigus vulgaris antigen or Cadherin family member 6. Desmoglein-3 is a 999 amino acid ~125kDa single pass type I transmembrane glycoprotein, a core component of the desmosome cell-cell junction between epithelial cells.

      Desmoglein-3 is synthesized in the basal and lower suprabasal layers of the skin.

      Mouse anti Human Desmoglein 3 antibody, clone 5G11 does not recognise desmoglein-1 or desmoglein-2.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Recombinant human desmoglein 3.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cytosol
      integral to membrane
      calcium ion binding
      homophilic cell adhesion
      desmosome
      cellular component disassembly involved in apoptosis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting1/1001/1000
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. pepsin (See Wu et al). Alternatively it is reported that staining of FFPE material may be enhanced using protein digestion with e.g. pronase, followed by HIER in citrate buffer.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting1/1001/1000
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. pepsin (See Wu et al). Alternatively it is reported that staining of FFPE material may be enhanced using protein digestion with e.g. pronase, followed by HIER in citrate buffer.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Skin
    • Histology Positive Control Tissue
      Skin.
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA2273 detects a band of approximately 125kDa in epithelial cell lysates.
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Desmoglein 3 Antibody Formats

    Formats Clone Applications Sizes available
    Desmoglein 3 Antibody : Purified 5G11 C, IP, P *, WB 25 µg | 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Skin
              Skin.

              References

              Further Reading

              Write your review

              You may also be interested in...

              You May Also Be Interested In...