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Desmoglein 2 antibody | 6D8

Mouse anti Human Desmoglein 2

Product Type
Monoclonal Antibody
Clone
6D8
Isotype
IgG1
Specificity
Desmoglein 2

Product Code Applications Pack Size List Price Your Price Qty
MCA2272T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IP WB 25 µg loader
List Price Your Price
loader
MCA2272
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IP WB 0.2 mg loader
List Price Your Price
loader

Mouse anti Human desmoglein 2 monoclonal antibody, clone 6D8 recognizes human desmoglein 2, a single pass type 1 membrane glycoprotein with 4 extracellular cadherin domains and six cytoplasmic desmoglein repeat sequences, with a predicted molecular weight of ~122 kDa and an apparent molecular weight of ~160-165 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between epithelial cells.

Mouse anti human desmoglein 2, clone 6D8 has been used for the study of the interaction between desmoglein 2 and adenoviruses which exploit desmoglein 2 as a receptor for infection (Wang et al. 2013).

Mouse anti Human desmoglein 2 monoclonal antibody, clone 6D8 recognizes a region encompassing a portion of extracellular domains 3 and 4 (Kolegraff et al. 2011) and does not recognize desmoglein-1 or desmoglein-3 (Wahl et al. 2002).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
A-431 cell membranes.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human skin
Western Blotting
Mouse anti Human desmoglein 2 antibody, clone 6D8 detects a band of approximately 165 kDa in A-431 cell lysates.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Desmoglein 2 antibody

  1. Ishii, K. et al. (2001) Assembly of desmosomal cadherins into desmosomes is isoform dependent.
    J Invest Dermatol. 117 (1): 26-35.
  2. Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
    Hybrid Hybridomics. 21 (1): 37-44.
  3. Sobolik-Delmaire, T. et al. (2006) Carboxyl terminus of Plakophilin-1 recruits it to plasma membrane, whereas amino terminus recruits desmoplakin and promotes desmosome assembly.
    J Biol Chem. 281 (25): 16962-70.
  4. Hemmoranta, H. et al. (2006) Transcriptional profiling reflects shared and unique characters for CD34+ and CD133+ cells.
    Stem Cells Dev. 15: 839-51.
  5. Nava, P. et al. (2007) Desmoglein-2: a novel regulator of apoptosis in the intestinal epithelium.
    Mol Biol Cell. 18: 4565-78.
  6. Yamamoto, Y. et al. (2007) Anti-desmoglein 3 (Dsg3) monoclonal antibodies deplete desmosomes of Dsg3 and differ in their Dsg3-depleting activities related to pathogenicity.
    J Biol Chem. 282: 17866-76.
  7. Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
    Hybridoma (Larchmt). 27 (4): 249-58.
  8. Todorović, V. et al. (2010) Detection of differentially expressed basal cell proteins by mass spectrometry.
    Mol Cell Proteomics. 9 (2): 351-61.
  9. View The Latest Product References
  10. Gornowicz-Porowska, J. et al. (2011) Loss of correlation between intensities of desmoglein 2 and desmoglein 3 expression in basal cell carcinomas.
    Acta Dermatovenerol Croat. 19: 150-5.
  11. Brennan, D. et al. (2012) A role for caveolin-1 in desmoglein binding and desmosome dynamics.
    Oncogene. 31 (13): 1636-48.
  12. Wang, H. et al. (2012) A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.
    J Virol. 86 (11): 6286-302.
  13. Wang, H. et al. (2013) Structural and functional studies on the interaction of adenovirus fiber knobs and desmoglein 2.
    J Virol. 87 (21): 11346-62.
  14. Pietkiewicz, P. et al. (2014) Discordant expression of desmoglein 2 and 3 at the mRNA and protein levels in nodular and superficial basal cell carcinoma revealed by immunohistochemistry and fluorescent in situ hybridization.
    Clin Exp Dermatol. 39 (5): 628-35.
  15. Wang, H. et al. (2015) Intracellular Signaling and Desmoglein 2 Shedding Triggered by Human Adenoviruses Ad3, Ad14, and Ad14P1.
    J Virol. 89 (21): 10841-59.
  16. Yumul, R. et al. (2016) Epithelial Junction Opener Improves Oncolytic Adenovirus Therapy in Mouse Tumor Models.
    Hum Gene Ther. 27 (4): 325-37.
  17. Kim, J. et al. (2020) Desmoglein-2 as a prognostic and biomarker in ovarian cancer.
    Cancer Biol Ther. : 1-9.

Immunofluorescence

Immunoprecipitation

Western Blotting

RRID
AB_2093429
UniProt
Q14126
Entrez Gene
DSG2
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0030057 desmosome

MCA2272T

166667 200514

MCA2272

154440 165876

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