Desmoglein 2 Antibody | 6D8

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  • Mouse anti Human Desmoglein 2
  • Mouse anti Human Desmoglein 2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    6D8
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2272C, IP, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2272
    MCA2272TC, IP, WBdatasheet pdfdatasheet pdf25 µg
    MCA2272T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human desmoglein 2 monoclonal antibody, clone 6D8 recognizes human desmoglein 2, a single pass type 1 membrane glycoprotein with 4 extracellular cadherin domains and six cytoplasmic desmoglein repeat sequences, with a predicted molecular weight of ~122 kDa and an apparent molecular weight of ~160-165 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between epithelial cells.

      Mouse anti human desmoglein 2, clone 6D8 has been used for the study of the interaction between desmoglein 2 and adenoviruses which exploit desmoglein 2 as a receptor for infection (Wang et al. 2013).

      Clone 6D8 does not recognize desmoglein-1 or desmoglein-3 (Wahl et al. 2002).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      A-431 cell membranes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      desmosome
      cellular component disassembly involved in apoptosis
      integral to membrane
      homophilic cell adhesion
      calcium ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting1/1001/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Skin
    • Histology Positive Control Tissue
      Skin
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA2272 detects a band of approximately 165kDa in A-431 cell lysates.
    • Western Blotting
      MCA2272 detects a band of approximately 165kDa in A-431 cell lysates.
    • Instructions For Use
    • Instructions For Use

    Additional Desmoglein 2 Antibody Formats

    Formats Clone Applications Sizes available
    Desmoglein 2 Antibody : Purified 6D8 C, IP, WB 0.2 mg | 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
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      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
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      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
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      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Skin
              Skin
              • Application NameReference Images
                Immunoprecipitation
                Western Blotting

              References

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