Desmin Antibody (PrecisionAbTM Antibody) | 4G1

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Desmin Antibody (PrecisionAb<sup>TM</sup> Antibody) | 4G1 gallery image 1

Western blot analysis of whole cell lysates probed with desmin antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 18 second exposure. Arrow points to desmin (molecular weight 53 kDa)

  • Desmin Antibody (PrecisionAb<sup>TM</sup> Antibody) | 4G1 thumbnail image 1
  • Mouse anti Desmin
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    Desmin antibody recognizes desmin, also known as intermediate filament protein or mutant desmin p.K241E. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00130WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Mouse anti Human desmin antibody recognizes desmin, also known as intermediate filament protein.

        The DES gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in DES are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies (provided by RefSeq, Jul 2008).

        Mouse anti Human desmin antibody detects a band of 53 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
      • Western Blotting
        Anti Desmin detects a band of approximately 53 kDa in C2C12 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from ascites.
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Fragment corresponding to amino acids 104-391 of human Desmin (NP_001918) produced in E. coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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                • View more products with "DESMIN" specificity.