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Desmin Antibody | 3744

Desmin Antibody | 3744 gallery image 1

Staining of paraffin processed normal human appendixWith Human anti Human Desmin (HCA023)

Desmin Antibody | 3744 gallery image 2

Western blot analysis of C2C12 whole cell lysate probed with Human anti Desmin (HCA023) at 1/200 (2.5μg/ml) followed by detection with HRP conjugated Mouse anti Histidine tag (MCA1396P ) at 1/1000 (1μg/ml). Arrow points to Desmin (molecular weight 55kDa)

  • Desmin Antibody | 3744 thumbnail image 1
  • Desmin Antibody | 3744 thumbnail image 2
  • Human anti Human Desmin
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA023C, E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • HCA023 is specific for human Desmin, which belongs to the class III family of intermediate filaments. Desmin constitutes part of the cytoskeleton, and is the characteristic intermediate filament of all three types of muscle cells (skletetal, cardiac and smooth muscle).
      Desmin is a 52-kDa protein encoded by nine exons of a gene located at 2q35. Desmin forms a cytosekeltal network across the muscle fibre bordering at the plasma and nuclear membrane and is particularly localized to the subplamalemmal region and the Z-band. Staining for Desmin is useful for identification of rhabdomyosarcomas and leiomyomas.

      For use in immunohistology staining we recommend the use of anti-His tag bridging antibody, followed by visualisation using the Dako Duet staining system
    • Intended Use
    • Target Species
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
    • Reconstitution
    • Preparation
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Recombinant human desmin produced in E. Coli
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/1001/500
      Western Blotting1/2001/2000
      Immunohistology - Paraffin(1)1/1001/500
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Desmin Antibody Formats

    Formats Clone Applications Sizes available
    Desmin Antibody : Purified 3744 C, E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Desmin Antibody | 3744 thumbnail image 1
                • Desmin Antibody | 3744 thumbnail image 2

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