Dendritic Cells/B Cells Antibody | RFD1

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  • Mouse anti Human Dendritic Cells
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  • Product Type
    Monoclonal Antibody
  • Clone
    RFD1
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1121C *datasheet pdfdatasheet pdf0.2 mg
    MCA1121
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human dendritic cells antibody, clone RFD1 recognizes an epitope within the class II MHC complex. In normal tissue mouse anti human dendritic cells antibody, clone RFD1 identifies dendritic cells and a proportion of B lymphocytes. Within the macrophage/dendritic cell populations the epitope seen by RFD1 is co-expressed with RFD7 by a subset of cells that exhibit suppressive activity on T cell stimulation.Mouse anti Human dendritic cells antibody, clone RFD1 does not bind to monocytes or cells of the granulocyte lineage.

      Mouse anti Human dendritic cells antibody, clone RFD1 has been used for the induction of apoptosis via MHC Class II cross linking of gastric mucosa epithelial cells (Fan et al. 1998).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Macaqueyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgM- liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by affinity chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Dendritic Cells/B Cells Antibody Formats

    Formats Clone Applications Sizes available
    Dendritic Cells/B Cells Antibody : Purified RFD1 C * 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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