Defensin beta 3 (BD-3) is a cationic non-haemolytic antimicrobial peptide and member of the beta-defensin family, highly expressed by skin and tonsils, which plays an important role in the innate immune response to potentially pathogenic microbes, including multiresistant S. aureus and vancomycin-resistant E. faecium.
Purified recombinant protein - lyophilised
Reconstitute with 20ul 10mM acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. N.B. For long term storage, further dilutions may be made in buffer containing 0.1% bovine serum albumin (BSA).
Purified recombinant defensin beta 3 expressed in E.coli
Exhibits chemotactic activity on monocytes.
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
5.1kD (45 amino acid residues)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.