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Defensin Beta 2

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  • Recombinant Human Defensin Beta 2
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP161E, FN, WBdatasheet pdfdatasheet pdf20 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant human defensin beta-2 (BD-2), is a 4.3 kDa peptide comprised of 41 amino acid residues. BD-2, a member of the beta defensin family, is secreted at epithelial surfaces of the skin and respiratory tract and by some leukocytes. This defensin is induced by bacterial products and cytokines during inflammation and functions as part of the innate immune system, having a wide ranging antimicrobial activity. BD-2 also functions as a chemoattractant to immature dendritic cells and memory T cells and acts as a ligand for TLR4, upregulating co-stimulatory molecules and inducing dendritic cell maturation.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.2ml 10mM acetic acid.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage the addition of 0.1% bovine serum albumin (BSA) is recommended.
    • Preparation
      Purified recombinant BD-2, expressed in E. coli
    • Preservative Stabilisers
      None present
    • Peptide Sequence
    • Activity
      Determined by its ability to chemoattract immature dendritic cells using a concentration of 10.0-100.0 ng/ml.
    • Purity
      >98% by SDS PAGE and HPLC analysis.
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
    • Molecular Weight
      4.3kD (41 amino acid sequence/residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      immune response
      extracellular region
      defense response to bacterium
      G-protein coupled receptor protein signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays10100ng/ml
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human BD-2 may be used as a standard in ELISA applications with either a purified human BD-2 antibody (AHP849) or a biotinylated human BD-2 antibody (AHP489B).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant human BD-2 may be used as the positive control in Western Blotting applications with either a purified human BD-2 antibody (AHP849) or a biotinylated human BD-2 antibody (AHP489B).
    • Instructions For Use

    Additional Defensin Beta 2 Formats

    Formats Applications Sizes available
    Defensin Beta 2 : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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