DDB1 Antibody

DDB1 Antibody gallery image 1

Western blot analysis of DDB1 in NSO cell lysate with Goat anti DDB1 antibody (AHP632)

DDB1 Antibody gallery image 2

Western blotting analysis of HeLa (A), HepG2 (B) and Jurkat (C) lysate (35μg protein in RIPA buffer) probed with Goat anti Human DDB1 antibody (AHP632) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

DDB1 Antibody gallery image 3

Western blotting of NIH3T3 (A) and NSO (B) lysate. (35μg protein in RIPA buffer) probed with Goat anti Human DDB1 antibody (AHP632) at 0.01μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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  • Goat anti DDB1 (C-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP632WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti DDB1 antibody recognizes human DNA damage-binding protein 1, also known as DDB p127 subunit, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-damaged DNA-binding protein 1, XPE-binding factor or Xeroderma pigmentosum group E-complementing protein. DDB1 is an 1140 amino acid ~ kDA cytoplasmic protein that translocated to the nucleus following UV irradiation and subsequently accumulates at the site of DNA damage wjere it forms part of the UV-DDB complex and is involved in the nucleotide-excision repair of DNA following damage caused by ultraviolet radiation.

      Goat anti DDB1 antibody binds to an epitope within the C-terminal (CT) region of damage-specific DNA binding protein 1.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to DDB1 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence DLIKVVEELTRIH corresponding to the C-terminal region of DDB1 (NP_001914).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      interspecies interaction between organisms
      damaged DNA binding
      protein binding
      nucleotide-excision repair, DNA damage removal
      proteasomal ubiquitin-dependent protein catabolic process
      Cul4B-RING ubiquitin ligase complex
      protein ubiquitination involved in ubiquitin-dependent protein catabolic process
      cell cycle checkpoint
      Cul4A-RING ubiquitin ligase complex
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.2ug/ml1ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP632 detects a band of approximately 130kDa in HeLa, Jurkat and NSO (mouse) cell lysates.
    • Instructions For Use

    Additional DDB1 Antibody Formats

    Formats Applications Sizes available
    DDB1 Antibody : Purified WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


              Further Reading

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