Darpp-32 Antibody (PrecisionAbTM Antibody)

Darpp-32 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of tissue lysate probed with DARPP-32 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to DARPP-32 (molecular weight 32 kDa)

  • Darpp-32 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti Darpp-32
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00687WBdatasheet pdfdatasheet pdf100 µl
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    • Rabbit anti Human DARPP-32 antibody recognizes the protein phosphatase 1 regulatory subunit 1B also known as dopamine and cAMP-regulated neuronal phosphoprotein 32.

      The PPP1R1B gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, PPP1R1B may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for PPP1R1B (provided by RefSeq, Oct 2011).

      Rabbit anti Human DARPP-32 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using mouse brain tissue lysate.
    • Target Species
    • Western Blotting
      Rabbit anti DARPP-32 detects a band of approximately 32 kDa in mouse brain tissue
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Preparation
      Rabbit polyclonal antibody purified by affinity chromatography on Protein A
    • Buffer Solution
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide
    • Immunogen
      KLH conjugated synthetic peptide encompassing part of human DARPP-32 (amino acids 106 - 139)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
    • Lysate Reconstitution
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      signal transduction
      protein phosphatase inhibitor activity
      protein kinase inhibitor activity
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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