DAI Antibody

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DAI Antibody gallery image 1

Western blot analysis of mouse small intestine lysate probed with Rabbit anti Human DAI (AHP1780) at 0.5(A), 1(B) and 2(C)

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DAI Antibody gallery image 2

Formalin fixed, paraffin embedded human small intestine stained with Rabbit anti Human DAI (AHP1780) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • DAI Antibody thumbnail image 1
  • DAI Antibody thumbnail image 2
  • Rabbit anti DAI
  • Rabbit anti DAI
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1780E, P *, WB*datasheet pdfdatasheet pdf0.1 mg
    AHP1780
    AHP1780TE, P *, WB*datasheet pdfdatasheet pdf50 µg
    AHP1780T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • This product recognizes human DAI, also known as Z-DNA Binding Protein (ZBP1), a ~46 kDa DNA binding protein belonging to a family of proteins that contain a Za domain which specifically binds to the left-handed Z-DNA and Z-RNA.

      DAI may be involved in processes such host response to neoplasia. cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK).

      DAI is highly expressed in lymphatic tissues such as lymph nodes, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seen in thymus, lung and liver. Multiple isoforms of DAI exist.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human DAI were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.02% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to an 18 amino acid sequence from near the carboxy terminus of human ZBP1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      double-stranded RNA adenosine deaminase activity
      RNA binding
      left-handed Z-DNA binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Paraffin(1)
      Western Blotting(2)0.51.0ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
      (2)
      Reduced and denatured conditions
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Paraffin(1)
      Western Blotting(2)0.51.0ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
      (2)
      Reduced and denatured conditions

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AHP1780T detects a band of approximately 46 kDa in mouse small intestine tissue lysate
    • Western Blotting
      AHP1780 detects a band of approximately 46 kDa in mouse small intestine tissue lysate
    • Instructions For Use
    • Instructions For Use

    Additional DAI Antibody Formats

    Formats Applications Sizes available
    DAI Antibody : Purified E, P *, WB* 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A

          Recommended Positive Controls

            Histology Controls

              References

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