Cytokeratin 20 Antibody (PrecisionAbTM Antibody)

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Cytokeratin 20 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with cytokeratin 20 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to cytokeratin 20 (molecular weight 44 kDa)

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  • Cytokeratin 20 Antibody with Control Lysate
  • Human anti Cytokeratin 20
  • Cytokeratin 20 Antibody with Control Lysate
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    Anti Human cytokeratin 20 antibody recognizes keratin, type I cytoskeletal 20, also known as KRT20, K20, CD20, cytokeratin-20, CK20 or keratin-20. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      HuCAL Fab bivalent
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00451WBdatasheet pdfdatasheet pdf100 µl
      VMA00451
      VMA00451KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00451K
      VMA00451KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00451KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Human anti Human cytokeratin 20 antibody recognizes cytokeratin 20, also known as KRT20, keratin, type I cytoskeletal 20 and keratin-20, protein IT.

        The protein encoded by the KRT20 gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21 (provided by RefSeq, Jul 2008).

        Human anti Human cytokeratin 20 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti cytokeratin 20 detects a band of approximately 44 kDa in MCF7 cell lysates
        Anti cytokeratin 20 detects a band of approximately 44 kDa in MCF7 cell lysates
        Anti cytokeratin 20 detects a band of approximately 44 kDa in MCF7 cell lysates
      • Product Form
        Purified IgG - liquid
        A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
        Pack Size: 2 Westerns20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
        Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
        Pack Size: 10 Westerns100μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
        Pack Size: 2 Westerns20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant human cytokeratin 20
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg MCF7 lysate lyophilized in RIPA buffer
        400μg MCF7 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        Golgi apparatus
        structural constituent of cytoskeleton
        intermediate filament organization
        intermediate filament
        protein binding
        apoptosis
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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