Cytokeratin 18 Antibody | CY90
Paraffin section of human colon adenocarcinoma (HIER citrate pH6 antigen retrieval) blocked with 10% FCS and stained with mouse anti human cytokeratin 18 (MCA1864H) and detected with goat anti mouse IgG:A488 (STAR117D488GA). The tissue was also counterstained with PureBlu Hoechst 33342 (135-1304)
Enlarge- Mouse anti Human Cytokeratin 18
- Mouse anti Human Cytokeratin 18
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Product TypeMonoclonal Antibody
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CloneCY90
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IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
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Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Human Cytokeratin 18 antibody, clone CY90 recognizes human cytokeratin 18, a 45 kDa member of the acidic family of Cytokeratins. Cytokeratin 18 is often expressed in combination with cytokeratin 8. This antibody stains a range of simple epithelial, but not stratified squamous epithelia.
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Intended Use
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Target SpeciesHuman
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Product FormReady To UseReady To Use
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Reconstitution
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Preparation
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Preservative Stabilisers
0.09% Sodium Azide 0.5% Bovine Serum Albumin 0.09% Sodium Azide 0.5% Bovine Serum Albumin -
ImmunogenHuman A-431 and MCF-7 cell lines
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Purity
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Approx. Protein Concentrations
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered saline
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StorageStore at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.18 months from date of despatch.
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GO Termsperinuclear region of cytoplasmcentriolar satelliteinterspecies interaction between organismsnegative regulation of apoptosiscell cycleGolgi to plasma membrane CFTR protein transportanatomical structure morphogenesiskeratin filamentstructural molecule activityprotein binding
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Immunofluorescence Immunohistology - Frozen Neat Immunohistology - Paraffin(1) Neat (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A is recommended for this purpose.Application Name Yes No Min Dilution Max Dilution Immunofluorescence Immunohistology - Frozen Neat Immunohistology - Paraffin(1) Neat (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A is recommended for this purpose.- Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Histology Positive Control TissueIntestine
- Histology Positive Control TissueIntestine
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Instructions For Use
- Instructions For Use
Additional Cytokeratin 18 Antibody Formats
| Formats | Clone | Applications | Sizes available |
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| Cytokeratin 18 Antibody : Ready To Use | CY90 | C, IF, P* | 1 ml | 5 ml |
Recommended Secondary Antibody
Recommended Negative Isotype Control
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | BUF025A |
Recommended Positive Controls
Histology Controls
Intestine
Intestine
References
1. MacFarlane, M. et al. (2000) Active caspases and cleaved cytokeratins are sequestered into cytoplasmic inclusions in TRAIL-induced apoptosis.J Cell Biol. 148 (6): 1239-54.
2. Holliday, D. et al. (2009) Novel multicellular organotypic models of normal and malignant breast: tools for dissecting the role of the microenvironment in breast cancer progression.Breast Cancer Res 11: R3
3. Clark, S.E. et al. (2011) Molecular subtyping of DCIS: heterogeneity of breast cancer reflected in pre-invasive disease.Br J Cancer. 2011 Jan 4;104(1):120-7
4. Ginzberg, H.H. et al (2001) Neutrophil-mediated epithelial injury during transmigration: role of elastase.Am J Physiol Gastrointest Liver Physiol. 281: G705-17.
