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Cytokeratin 17 Antibody | 2D4-1G9

Cytokeratin 17 Antibody | 2D4-1G9 gallery image 1

Immunofluorescence showing Cytokeratin 17 in HeLa cells using Mouse anti Human Cytokeratin 17:Azide Free (MCA3226Z)

Cytokeratin 17 Antibody | 2D4-1G9 gallery image 2

Staining of paraffin embedded human tonsil with Mouse anti Human Cytokeratin 17:Azide Free (MCA3226Z)

Cytokeratin 17 Antibody | 2D4-1G9 gallery image 3

Western blot analysis of Cytokeratin 17 expression in A-431 using Mouse anti Human Cytokeratin 17:Azide Free (MCA3226Z)

  • Cytokeratin 17 Antibody | 2D4-1G9 thumbnail image 1
  • Cytokeratin 17 Antibody | 2D4-1G9 thumbnail image 2
  • Cytokeratin 17 Antibody | 2D4-1G9 thumbnail image 3
  • Mouse anti Human Cytokeratin 17:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3226ZIF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human cytokeratin 17 antibody, clone 2D4-1G9 recognizes human Keratin, type I cytoskeletal 17, also known as cytokeratin 17, 39.1 or Keratin-17. Cytokeratin 17 is a 432 amino acid ~48 kDa protein essential for hair follicle development and involved in shaping and orientation of hair.

      Mutations of the KRT17 gene can lead to development of an autosomal dominant ectodermal dysplasia “Pachyonychia congenita 2” (PC2) presenting with hypertrophic nail dystrophy, or "Steatocystoma multiplex" (SM) characterised by the development of cystic tumors on the back, thighs, trunk, arms or thighs.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to amino acids 1-433 of human Cytokeratin 17, with a GST tag.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting15ug/ml
      Immunohistology - Paraffin(1)0.110ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Western Blotting
      MCA3226Z detects a band of approximately 48 kDa in A431 cell lysates.
    • Instructions For Use

    Additional Cytokeratin 17 Antibody Formats

    Formats Clone Applications Sizes available
    Cytokeratin 17 Antibody : Preservative Free 2D4-1G9 IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human tonsil
                • Product Images

                • Cytokeratin 17 Antibody | 2D4-1G9 thumbnail image 1
                • Cytokeratin 17 Antibody | 2D4-1G9 thumbnail image 2
                • Cytokeratin 17 Antibody | 2D4-1G9 thumbnail image 3


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