Cytokeratin 14 Antibody | AbD03759

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Cytokeratin 14 Antibody | AbD03759 gallery image 1

Human anti Human Cytokeratin 14, clone AbD03759 Western blot
Lane 1: Western blot carried out using Human anti Human cytokeratin 14 antibody (HCA078) at 0.5 µg/ml on Hela whole cell lysate. For detection, HRP conjugated Mouse anti-Histidine tag (MCA5995P ) was applied at 1/5000. Image was visualized on the Bio-Rad Chemidoc Imager

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Cytokeratin 14 Antibody | AbD03759 gallery image 2

Human anti Human Cytokeratin 14 (clone AbD03759) Western blot
Lane 1: Western blot carried out using Human anti Human Cytokeratin 14 antibody (HCA078) at 0.5 µg/ml on Hela whole cell lysate. For detection, HRP conjugated Goat anti-Human IgG F(ab')2 antibody (STAR126P ) was applied at 1/2500. Image was visualized on the Bio-Rad Chemidoc Imager

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  • Human anti Human Cytokeratin 14
  • Human anti Human Cytokeratin 14
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    AbD03759
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA078E, P*, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA078
    HCA078AE, P*datasheet pdfdatasheet pdf50 µg
    HCA078A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human Cytokeratin 14 antibody, clone AbD03759 recognizes human cytokeratin 14 (CK14), a 50kDa acidic type I intermediate filament. The intermediate filaments are expressed as heterodimers in epithelial tissues. CK14 is expressed in the basal compartment of all stratified squamous epithelia and is co-expressed with cytokeratin 5 (CK5). Mutations in the cytokeratin 5 (KRT5) and cytokeratin 14 (KRT14) genes have been identified in patients with the skin disorder Epidermolysis bullosa simplex (EBS).Clone AbD03759 is also known under the designation clone 3759.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Pack Size: 0.1 mgA bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
      Pack Size: 50 µgA bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      Pack Size: 0.1 mgA bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
      Pack Size: 50 µgA bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      Pack Size: 0.1 mg0.01% Thiomersal
      Pack Size: 50 µg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 0.1 mg0.01% Thiomersal
      Pack Size: 50 µg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Recombinant human cytokeratin 14
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgTotal protein concentration 0.5 mg/ml
      Pack Size: 50 µgTotal protein concentration 0.1mg/ml
      Pack Size: 0.1 mgTotal protein concentration 0.5 mg/ml
      Pack Size: 50 µgTotal protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of despatch.
    • GO Terms
      cytosol
      keratin filament
      nucleus
      intermediate filament bundle assembly
      epidermis development
      mitochondrion
      structural constituent of cytoskeleton
      hemidesmosome assembly
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1000
      Immunohistology - Paraffin(1)1/251/50
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1000
      Immunohistology - Paraffin(1)1/251/50
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human bladder
    • Histology Positive Control Tissue
      Human bladder
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Cytokeratin 14 Antibody Formats

    Formats Clone Applications Sizes available
    Cytokeratin 14 Antibody : Purified AbD03759 E, P*, WB 0.1 mg | 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human bladder
              Human bladder

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