Cytochrome P450 Aromatase Antibody (PrecisionAbTM Antibody)

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Cytochrome P450 Aromatase Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with cytochrome P450 aromatase antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to cytochrome P450 aromatase (molecular weight 58 kDa).

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  • Cytochrome P450 Aromatase Antibody with Control Lysate
  • Rabbit anti Cytochrome P450 Aromatase
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    Cytochrome P450 aromatase antibody recognizes Cytochrome P450 aromatase, also known as CYP450 family 26 subfamily B member, cytochrome P450 retinoid metabolizing protein or cytochrome P450, family 26, subfamily B, polypeptide 1.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00769WBdatasheet pdfdatasheet pdf100 µl
      VPA00769
      VPA00769KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00769KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human cytochrome P450 aromatase antibody recognizes cytochrome P450 aromatase, also known as CYP450 family 26 subfamily B member, cytochrome P450 retinoid metabolizing protein or cytochrome P450, family 26, subfamily B, polypeptide 1.

        This gene encodes a member of the cytochrome P450 superfamily. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein is localized to the endoplasmic reticulum, and functions as a critical regulator of all-trans retinoic acid levels by the specific inactivation of all-trans retinoic acid to hydroxylated forms. Mutations in this gene are associated with radiohumeral fusions and other skeletal and craniofacial anomalies, and increased levels of the encoded protein are associated with atherosclerotic lesions. Alternative splicing results in multiple transcript variants (provided by RefSeq, Apr 2013).

        Rabbit anti Human cytochrome P450 aromatase antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti cytochrome P450 aromatase antibody recognizes a band of approximately 58 kDa in HepG2 cell lysates
        Anti cytochrome P450 aromatase antibody recognizes a band of approximately 58 kDa in HepG2 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
        Rabbit polyclonal antibody purified by affinity chromatography on immunogen
      • Buffer Solution
        TRIS buffered glycine
        TRIS buffered glycine
      • Preservative Stabilisers
        0.01% Thiomersal
        <20% Glycerol
        0.01% Thiomersal
        <20% Glycerol
      • Immunogen
        Recombinant protein encompassing a sequence within the center region of human cytochrome P450 aromatase
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        spermatogenesis
        retinoic acid 4-hydroxylase activity
        proximal/distal pattern formation
        endoplasmic reticulum membrane
        retinoic acid binding
        negative regulation of retinoic acid receptor signaling pathway
        electron carrier activity
        retinoic acid catabolic process
        oxidation-reduction process
        heme binding
        male meiosis
        embryonic limb morphogenesis
        xenobiotic metabolic process
        cell fate determination
        microsome
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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