Cytochrome P450 3A4 Antibody

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Rabbit anti Human cytochrome p450 3A4 antibody used for the detection of P450 3A4 expressing cells in liver sections by immunofluorescence.
Image caption:
Experimental timeframe and analysis of human hepatocyte engraftment in HUHEP and HIS-HUHEP mice. (A) Time course of the experiment indicating the age of the mice at each step. Human albumin (hAlb) plasma levels were analyzed longitudinally in HUHEP (n = 4) (B) and HIS-HUHEP (n = 5) (C) mice. (D, E, F and G) Immunofluorescence analysis of HUHEP (left) and HIS-HUHEP (right) liver sections for anti-hAlb (red stain) co-stained with either (D) anti-E-Cadherin (green stain), or (E) anti-Occludin (green stain), or (F) anti-human Cyp2C9 (green stain), or (G) anti-human Cyp3A4 (green stain) expression. Nuclei are stained with DAPI (blue). Scale bar represents 100 microm.

From: Strick-Marchand H, Dusséaux M, Darche S, Huntington ND, Legrand N, et al. (2015) A Novel Mouse Model for Stable Engraftment of a Human Immune System and Human Hepatocytes.
PLoS ONE 10(3): e0119820.

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Published customer image:
Rabbit anti Human p450 3A4 antibody used for the detection of p450 3A4 in human neuroblastoma lysates by western blotting
Image caption:
Expression of CYP1A1, 1B1 and 3A4 in human neuroblastoma cell lines UKF-NB-3, UKF-NB-4 and SK-N-AS by Western blot.

From: Hřebačková, J, Poljaková J, Eckschlager T, Hraběta J, Procházka P, Smutný S, Stiborová M. Histone deacetylase inhibitors valproate and trichostatin A are toxic to neuroblastoma cells and modulate cytochrome P450 1A1, 1B1 and 3A4 expression in these cells.
Interdiscip Toxicol. 2009 Sep;2(3):205-10.

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  • Rabbit anti Human Cytochrome P450 3A4
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP622ZFN, WBdatasheet pdfdatasheet pdf50 µl
    AHP622Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human cytochrome p450 3A4 antibody recognizes human cytochrome p450 3A4 (CYP3A4). P450 enzymes are divided into two groups: steroidogenic and xenobiotic. The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism. Work in this area is ongoing but studies suggest that all known drug metabolism is mediated by family members:CYP3A, CYP2D6, CYP1A2, CYP2C9/10, CYP2C19 and CYP2E1. Rabbit anti Human cytochrome p450 3A4 antibody is a neutralizing antibody, a specific and potent inhibitor of CYP3A4.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays2-20ul
      Western Blotting1/50001/10000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Cytochrome P450 3A4 Antibody Formats

    Formats Applications Sizes available
    Cytochrome P450 3A4 Antibody : Serum FN, WB 50 µl
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

              Further Reading

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