Cytochrome P450 2E1 Antibody (PrecisionAbTM Antibody) | OTI5F11

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Cytochrome P450 2E1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI5F11 gallery image 1

Western blot analysis of whole cell lysates probed with cytochrome P450 2E1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 89 second exposure. Arrow points to cytochrome P450 2E1 (molecular weight 57 kDa)

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  • Cytochrome P450 2E1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI5F11 thumbnail image 1
  • Mouse anti Cytochrome P450 2E1
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    Cytochrome P450 2E1 antibody recognizes cytochrome P450 2E1, also known as 4-nitrophenol 2-hydroxylase, CYPIIE1, subfamily IIE (ethanol-inducible), cytochrome P450-J, flavoprotein-linked monooxygenase or microsomal monooxygenase. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00156WBdatasheet pdfdatasheet pdf100 µl
      VMA00156
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human cytochrome P450 2E1 antibody recognizes cytochrome P450 2E1, also known as 4-nitrophenol 2-hydroxylase, CYPIIE1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, cytochrome P450-J, flavoprotein-linked monooxygenase, microsomal monooxygenase and xenobiotic monooxygenase.

        Encoded by the CYP2E1 gene, cytochrome P450 2E1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer (provided by RefSeq, Jul 2008).

        Mouse anti Human cytochrome P450 2E1 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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