Recombinant Human cystatin C is a prpparation of cystatin C madein E. coli. Cystatin C is a member of the type 2 cystatin superfamily. It is a potent inhibitor of cysteine proteinases, both endogenous (lysosomal cathepsins), and pathogenic. Cystatin C is a 13kDa secreted protein which is found in high concentrations in biological fluids and is thought to be expressed by all human nucleated cells.
Changes in cystatin C have been documented in a number of cardiovascular diseases, including atherosclerosis, amyloid angiopathy and myocardial infarction. Its low molecular weight also means that Cystatin C can be used as a marker of renal failure.
Recombinant Human cystatin C (PHP202) is a single polypeptide chain corresponding to mature secreted protein with an additional Met residue at the N-terminal.
Purified recombinant protein - lyophilised
Reconstitute with deionized water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant Cystatin C expressed in E.coli
>95% by SDS PAGE
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.