Cyclophilin A Antibody | 794

//www.bio-rad-antibodies.com/human-cyclophilin-a-antibody-794-hca005.html?RenderPageSpecific=1
Cyclophilin A Antibody | 794 gallery image 1

HeLa whole cell lysate probed with Human anti Human Cyclophilin A:Biotin (HCA005B)

Enlarge
Cyclophilin A Antibody | 794 gallery image 2

HeLa whole cell lysate probed with Human anti Human Cyclophilin A:HRP (HCA005P)

Enlarge
Cyclophilin A Antibody | 794 gallery image 3

HeLa lysate probed with Human anti Cyclophilin A: DyLight 800(HCA005D800) Imaged with the Licor Odyssey®SA

Enlarge
Cyclophilin A Antibody | 794 gallery image 4

Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Human anti Human cyclophilin A antibody (HCA005) followed by HRP conjugated F(ab')2 Goat anti Human IgG, visualized by chemiluminescence

Enlarge
  • Cyclophilin A Antibody | 794 thumbnail image 1
  • Cyclophilin A Antibody | 794 thumbnail image 2
  • Cyclophilin A Antibody | 794 thumbnail image 3
  • Cyclophilin A Antibody | 794 thumbnail image 4
  • Human anti Cyclophilin A
  • Human anti Cyclophilin A:DyLight®800
  • Human anti Cyclophilin A:DyLight®750
  • Human anti Cyclophilin A:DyLight®680
  • Human anti Cyclophilin A:Biotin
  • Human anti Cyclophilin A:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    794
  • Isotype
    HuCAL Fab bivalent
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA005E, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA005
    HCA005D800WBdatasheet pdfdatasheet pdf0.1 mg
    HCA005D800
    HCA005D750WBdatasheet pdfdatasheet pdf0.1 mg
    HCA005D750
    HCA005D680WBdatasheet pdfdatasheet pdf0.1 mg
    HCA005D680
    HCA005BE, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA005B
    HCA005PE, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA005P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Cyclophilin A antibody, clone 794 recognizes Cyclophilin A, also known as peptidyl-prolyl cis/trans isomerase A, PPIA or Rotamase A. Cyclophilin A is a ~18kDa, ubiquitously distributed intracellular protein that can be secreted by cells in response to inflammatory stimuli (Jin et al. 2000).

      Cyclophilin A is a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and, therefore, are believed to be involved in protein folding and/or intracellular protein transport (Fischer et al. 1989). This protein can interact with several HIV proteins including p55 gag, Vpr, and capsid protein. It has been shown to be necessary for the formation of infectious HIV virions (Franke et al. 1994). Cyclophilin is a specific high-affinity binding protein for the immunosuppressant agent cyclosporin A (Handschumacher et al. 1984). Because of its dramatic effects on decreasing morbidity and increasing survival rates in human transplants, the molecular mechanism of immunosuppression by cyclosporin A has been a matter of much interest (Durette et al. 1988). Multiple PPIA pseudogenes that map to different chromosomes have been reported. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed (Chatellard-Gruaz D et al. 1994).

      Mouse anti cyclophilin antibody, clone 794 has been used successfully for the detection of cyclophilin A in Hela cell lysates by Western blotting.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      PigExpected from Sequence
      MonkeyExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      A liquid bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight®800 - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight®750 - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight®680 - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to Biotin - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to horseradish peroxidase (HRP) - liquid.
    • Reconstitution
    • Preparation
      Metal-chelate affinity chromatography.
      Metal-chelate affinity chromatography.
      Metal-chelate affinity chromatography.
      Metal-chelate affinity chromatography.
      Metal-chelate affinity chromatography.
      Metal-chelate affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.01% Thiomersal
    • Immunogen
      Full length human recombinant cyclophilin A, amino acid residues 1-164.
      MW 17.9 kDa.
    • Activity
      Activity was tested by indirect ELISA: recombinant purified Cyclophilin A (5 μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA005 (2 μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate. 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above background.
    • Purity
      Purity was tested by SDS-PAGE and Coomassie-staining of a 2 µg sample.
    • Approx. Protein Concentrations
      Antibody concentration 0.5 mg/ml.
      Antibody concentration 1.0 mg/ml
      Antibody concentration 1.0 mg/ml
      Antibody concentration 1.0 mg/ml
      Antibody concentration 0.1mg/ml
      0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
      6 months from date of despatch.
      6 months from date of despatch.
      6 months from date of despatch.
      6 months from date of despatch.
      6 months from date of despatch.
    • GO Terms
      leukocyte migration
      entry into host cell
      protein folding
      virion binding
      uncoating of virus
      peptide binding
      peptidyl-prolyl cis-trans isomerase activity
      platelet degranulation
      RNA-dependent DNA replication
      nucleus
      cytosol
      regulation of viral genome replication
      platelet activation
      provirus integration
      extracellular region
      unfolded protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA2ug/ml
      Western Blotting0.5ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/10001/5000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
      This product is suitable for use as a loading control in Western Blotting
    • Western Blotting
      This product is suitable for use as a loading control in Western Blotting
    • Western Blotting
      This product is suitable for use as a loading control in Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Cyclophilin A Antibody Formats

    Formats Clone Applications Sizes available
    Cyclophilin A Antibody : Biotin 794 E, WB 0.1 mg
    Cyclophilin A Antibody : DyLight®680 794 WB 0.1 mg
    Cyclophilin A Antibody : Purified 794 E, WB 0.1 mg
    Cyclophilin A Antibody : DyLight®750 794 WB 0.1 mg
    Cyclophilin A Antibody : HRP 794 E, WB 0.1 mg
    Cyclophilin A Antibody : DyLight®800 794 WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      0500-0100
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      0500-0100
      Mouse anti Histidine Tag:HRPMCA1396P0.1 mgE, WB
      MCA1396P
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      0500-0100
      Mouse anti Histidine Tag:HRPMCA1396P0.1 mgE, WB
      MCA1396P
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      0500-0100
      Mouse anti Histidine Tag:HRPMCA1396P0.1 mgE, WB
      MCA1396P
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...