CXCL9 Antibody

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CXCL9 Antibody gallery image 1

Formalin fixed, paraffin embedded human renal tumor with parenchymal tissue stained with Rabbit anti Human CXCL9 (AHP1864) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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CXCL9 Antibody gallery image 2

Formalin fixed, paraffin embedded human renal tumor with parenchymal tissue stained with Rabbit anti Human CXCL9 (AHP1864) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • CXCL9 Antibody thumbnail image 1
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  • Rabbit anti Human CXCL9
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1864E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1864
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human CXCL9 polyclonal antinbody recognizes human CXCL9, otherwise known as MIG (monokine induced by Interferon-gamma), a member of the CXC family of chemokine receptors, secreted by interferon stimulated monocytes, macrophages (Muller et al. 2010) and endothelial cells (Loos et al. 2006) CXCL9 signals through binding to CD183 (CXCR3).

      CXCL9 acts as a Th1 (type 1 helper T) cell chemoattractant, and plays a role in the growth, activation and movement of cells associated with immune and inflammatory responses, and in tumour growth inhibition and angiogenesis (Whiting et al. 2004) . Studies have shown that CXCL9 is active against E.coli, S.aureus, L.monocytogenes (Cole et al. 2001) and S.pyogenes (Egesten et al. 2007).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antiserum to human CXCL9 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant human CXCL9
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      cell-cell signaling
      inflammatory response
      extracellular space
      immune response
      cellular defense response
      G-protein coupled receptor protein signaling pathway
      chemotaxis
      chemokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays5.07.0ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CXCL9 Antibody Formats

    Formats Applications Sizes available
    CXCL9 Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              References

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