CSDA Antibody | 1H5

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CSDA Antibody | 1H5 gallery image 1

Western Blot analysis of CSDA expression in HeLa using Mouse anti Human CSDA (MCA5019Z)

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CSDA Antibody | 1H5 gallery image 2

Immunofluorescence showing CSDAin HeLa cells using Mouse anti Human CSDA (MCA5019Z)

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  • CSDA Antibody | 1H5 thumbnail image 1
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  • Mouse anti Human CSDA:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    1H5
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5019ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5019Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CSDA antibody, clone 1H5 recognizes human Y-box-binding protein 3, also known as CSDA, Cold shock domain-containing protein A, DNA-binding protein A or Single-strand DNA-binding protein NF-GMB. CSDA is a 371 amino acid ~30-40 kDa repressor protein binding to the GM-CSF receptor.

      Three isoforms of CSDA, generated by alternative splicing have been described. Mouse anti Human CSDA antibody, clone 1H5 binds to the canonical isoform 1, it would also be expected to recognize the c-terminally truncated isoform 3 as this contains the complete immunogen sequence used to isolate clone 1H5. It is not clear whether the antibody will recognize the truncated isoform 2 as this lacks part of the immunogen sequence used to raise Mouse anti Human CSDA antibody, clone 1H5 (UniProt : P16989).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 241-331 of human Y-box-binding protein 3.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      sequence-specific DNA binding transcription factor activity
      response to cold
      double-stranded DNA binding
      negative regulation of transcription from RNA polymerase II promoter
      cytoplasm
      nucleus
      transcription corepressor activity
      RNA polymerase II transcription factor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CSDA Antibody Formats

    Formats Clone Applications Sizes available
    CSDA Antibody : Preservative Free 1H5 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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