Goat anti Human CRMP2 antibody recognises the carboxy-terminal region of human collapsin response mediator protein 2 (CRMP2), a ubiquitously expressed, ~62 kDa member of the dehydropyrimidinase family. It is involved in axon formation and outgrowth by transporting to axons, the actin cytoskeleton regulating; Rac1-associated protein 1 (Sra-1)/WASP family verprolin-homologous protein 1 (WAVE1) complex.
CRMP2 also regulates axon formation and branching by binding tubulin heterodimers thereby enhancing microtubule polymerisation. Through its interaction with ROKalpha, CRMP2 is also thought to play a role in RhoA-dependent signalling. CRMP2 is associated with mesial temporal lobe epilepsy and schizophrenia.
Serum - liquid
Antisera to CRMP2 (C-terminal) were raised by repeated immunisations of goats with highly purified antigen.
Sodium Azide (NaN3)
Synthetic peptide corresponding amino acid sequence 543-557 of human CRMP2.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1255 detects bands of approximately 62 and 124kDa in human Alzheimer disease brain cell lysates.