Western blotting analysis of K562 lysate (35µg protein in RIPA buffer) probed with Goat anti Human CRKL antibody (AHP693) at 0.01μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Goat anti Human CRKL antibody recognizes CRKL, a ~33 kDa adaptor protein shown to activate the RAS and JUN kinase signalling pathways and transform fibroblasts in a RAS-dependant manner.
CRKL is a substitute of BCR-ABL tyrosine kinase.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antisera to CRKL were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Bovine Serum Albumin
Synthetic peptide sequence KIFDPQNPDENE derived from C-terminus of CRKL.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP693 detects a band of approximately 33kDa in mouse kidney cell lysates. Overnight incubation with primary antibody is recommended.